College of Food Science, Shenyang Agricultural University, Shenyang 11086, China.
College of Science, Shenyang Agricultural University, Shenyang 11086, China.
Food Chem. 2023 Sep 1;419:136081. doi: 10.1016/j.foodchem.2023.136081. Epub 2023 Mar 30.
Milk fat globule membrane (MFGM) proteins are highly glycosylated and involved in various biological processes within the body. However, information on site-specific N-glycosylation of MFGM glycoproteins in donkey and human milk remains limited. This study aimed to map the most comprehensive site-specific N-glycosylation fingerprinting of donkey and human MFGM glycoproteins using a site-specific glycoproteomics strategy. We identified 1,360, 457, 2,617, and 986 site-specific N-glycans from 296, 77, 214, and 196 N-glycoproteins in donkey colostrum (DC), donkey mature milk (DM), human colostrum (HC), and human mature milk (HM), respectively. Bioinformatics was used to describe the structure-activity relationships of DC, DM, HC, and HM MFGM N-glycoproteins. The results revealed differences in the molecular composition of donkey and human MFGM N-glycoproteins and the dynamic changes to site-specific N-glycosylation of donkey and human MFGM glycoproteins during lactation, deepening our understanding of the composition of donkey and human MFGM N-glycoproteins and their potential physiological roles.
乳脂肪球膜（MFGM）蛋白高度糖基化，参与体内的各种生物学过程。然而，有关驴乳和人乳 MFGM 糖蛋白的特定部位 N-糖基化的信息仍然有限。本研究旨在使用特定部位糖蛋白质组学策略，绘制驴乳和人乳 MFGM 糖蛋白最全面的特定部位 N-糖基化指纹图谱。我们分别从驴初乳（DC）、驴成熟乳（DM）、人初乳（HC）和人成熟乳（HM）中鉴定出 296、77、214 和 196 个 N-糖蛋白中的 1360、457、2617 和 986 个特定部位 N-聚糖。使用生物信息学来描述 DC、DM、HC 和 HM MFGM N-糖蛋白的结构-活性关系。结果揭示了驴乳和人乳 MFGM N-糖蛋白的分子组成以及驴乳和人乳 MFGM 糖蛋白特定部位 N-糖基化在泌乳期间的动态变化存在差异，加深了我们对驴乳和人乳 MFGM N-糖蛋白的组成及其潜在生理作用的理解。
