Rykowski M C, Parmelee S J, Agard D A, Sedat J W
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0554.
Cell. 1988 Aug 12;54(4):461-72. doi: 10.1016/0092-8674(88)90067-0.
We have aligned the molecular map of the Notch locus to the cytological features of the salivary gland polytene chromosomes of D. melanogaster in order to determine the interphase chromatin structure of this gene. Using high-resolution in situ hybridization and computer-aided optical microscope data collection and image analysis, we have determined that the coding portions and introns of the Notch gene, which is not expressed in this tissue, are all contained within the polytene chromosome band 3C7. The portion of the Notch gene that resides 5' to the start of transcription lies in an open chromatin conformation, the interband between bands 3C6 and 3C7. Our data are most consistent with condensation of the chromosomal DNA into 30 nm fibers in this polytene band.
我们已将Notch基因座的分子图谱与黑腹果蝇唾液腺多线染色体的细胞学特征进行比对，以确定该基因在间期的染色质结构。通过高分辨率原位杂交以及计算机辅助光学显微镜数据收集和图像分析，我们确定在该组织中不表达的Notch基因的编码部分和内含子均包含在多线染色体带3C7内。Notch基因位于转录起始位点5'端的部分处于开放染色质构象，即3C6和3C7带之间的间带。我们的数据与该多线带中染色体DNA凝聚成30纳米纤维的情况最为相符。
