Friedman T B, Owens K N, Burnett J B, Saura A O, Wallrath L L
Graduate Program in Genetics, Michigan State University, East Lansing 48824.
Mol Gen Genet. 1991 Apr;226(1-2):81-7. doi: 10.1007/BF00273590.
Urate oxidase mRNA and five other transcripts map along 38 kb of DNA in the region 28C on the Drosophila melanogaster second chromosome. Three biotinylated restriction fragments from this 38 kb of DNA, one from each end and one from the middle, were individually hybridized in situ to slightly stretched salivary gland polytene chromosomes. The data from these in situ hybridizations in combination with the transcription map of the 38 kb of DNA indicate that: (i) there are six discrete RNA species encoded along the 38 kb of DNA and (ii) these six transcripts map to the faint band/interband region which includes the proximal edge of 28C1, the three faint bands, 28C2, 28C3 and 28C4-5(-), and the adjacent interband chromatin. Our data are consistent with the few published studies directly demonstrating that faint band/interband regions of the Drosophila melanogaster salivary gland polytene chromosomes code for a high density of transcripts.
尿酸氧化酶信使核糖核酸（Urate oxidase mRNA）和其他五种转录本沿果蝇（Drosophila melanogaster）第二条染色体上28C区域的38千碱基对（kb）DNA分布。从这38 kb DNA中选取的三个生物素化限制片段，一端一个，中间一个，分别原位杂交到轻度伸展的唾液腺多线染色体上。这些原位杂交的数据与38 kb DNA的转录图谱相结合表明：（i）沿38 kb DNA编码有六种离散的RNA种类；（ii）这六种转录本定位于微弱带/间带区域，该区域包括28C1的近端边缘、三条微弱带28C2、28C3和28C4 - 5（-）以及相邻的间带染色质。我们的数据与少数已发表的研究结果一致，这些研究直接表明果蝇唾液腺多线染色体的微弱带/间带区域编码高密度的转录本。
