Li Qi, Yin Jie, Ran Qing-Sen, Yang Qing, Liu Li, Zhao Zheng, Li Yu-Jie, Chen Ying, Sun Li-Dong, Wang Ya-Jie, Weng Xiao-Gang, Cai Wei-Yan, Zhu Xiao-Xin
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China School of Chinese Materia Medica, Capital Medical University Beijing 100069, China.
Zhongguo Zhong Yao Za Zhi. 2019 Jun;44(11):2317-2323. doi: 10.19540/j.cnki.cjcmm.20190210.001.
This paper was mainly to discuss the potential role and mechanism of Lianhua Qingwen Capsules(LHQW) in inhibiting pathological inflammation in the model of acute lung injury caused by bacterial infection. For in vitro study, the mRNA expression of MCP-1 in RAW264.7 cells and THP-1 cells, the content of MCP-1 in cell supernatant, as well as the effect of LHQW on chemotaxis of macrophages were detected. For in vivo study, mice were randomly divided into 7 groups, including normal group, model group(LPS 5 mg·kg(-1)), LHQW 300, 600 and 1 200 mg·kg(-1)(low, middle and high dose) groups, dexamethasone 5 mg·kg~(-1) group and penicillin-streptomycin group. Then, the anal temperature was detected two hours later. Dry weight and wet weight of lung tissues in mice were determined; TNF-α and MCP-1 levels in alveolar lavage fluid and MCP-1 in serum were detected. In addition, the infiltration of alveolar macrophages was also observed and the infiltration count of alveolar macrophages was measured by CCK-8 method. HE staining was also used to observe the inflammatory infiltration of lung tissues in mice. Both of the in vitro and in vivo data consistently have confirmed that: by down-regulating the expression of MCP-1, LHWQ could efficiently decrease the chemotaxis of monocytes toward the pulmonary infection foci, thus blocking the disease development in ALI animal model.
本文主要探讨连花清瘟胶囊（LHQW）在抑制细菌感染所致急性肺损伤模型中病理性炎症的潜在作用及机制。体外研究检测RAW264.7细胞和THP-1细胞中MCP-1的mRNA表达、细胞上清液中MCP-1的含量以及LHQW对巨噬细胞趋化性的影响。体内研究将小鼠随机分为7组，包括正常组、模型组（LPS 5 mg·kg⁻¹）、LHQW 300、600和1200 mg·kg⁻¹（低、中、高剂量）组、地塞米松5 mg·kg⁻¹组和青霉素-链霉素组。两小时后检测肛温，测定小鼠肺组织干重和湿重，检测肺泡灌洗液中TNF-α和MCP-1水平以及血清中MCP-1水平。此外，观察肺泡巨噬细胞浸润情况并采用CCK-8法测定肺泡巨噬细胞浸润计数，还用HE染色观察小鼠肺组织的炎性浸润。体外和体内数据均一致证实：通过下调MCP-1的表达，LHWQ可有效降低单核细胞向肺部感染灶的趋化性，从而阻断ALI动物模型中的疾病发展。
