Laboratório de Biologia Celular, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista "Júlio de Mesquita Filho", IBILCE/UNESP, São José do Rio Preto, Brazil.
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, UNICAMP-Avenida Bertrand Russel, Campinas, Brazil.
Am J Trop Med Hyg. 2019 Sep;101(3):605-607. doi: 10.4269/ajtmh.18-0650.
Chagas disease is a public health problem caused by the , and the I (TcI) and II (TcII) groups are considered important genotypes from the clinical point of view. Currently, the groups need to be molecularly analyzed for their identification; thus, we cytogenetically analyzed these groups with the objective of developing more accessible techniques for the characterization of these parasites. TcI and TcII groups were differentiated by nucleus characterization with lacto-acetic orcein (TcI-nucleus with positive heteropycnosis and TcII-nucleus with negative heteropycnosis), emphasizing the importance of the application of this technique for epidemiological and clinical studies of Chagas disease.
恰加斯病是一种公共卫生问题,由 引起,I (TcI)和 II (TcII)组被认为从临床角度来看是重要的基因型。目前,需要对这些组进行分子分析以进行鉴定;因此,我们对这些组进行了细胞遗传学分析,目的是开发更易于获得的技术来对这些寄生虫进行特征描述。通过乳-乙酸-地衣红(TcI-核阳性异固缩和 TcII-核阴性异固缩)对核进行特征描述来区分 TcI 和 TcII 组,强调了该技术在恰加斯病的流行病学和临床研究中的应用的重要性。