Puri Vandana, Chhikara Aruna, Sharma Geetika, Sehgal Shivali, Sharma Sunita
Department of Pathology, Lady Hardinge Medical College, New Delhi, India.
Asian J Transfus Sci. 2019 Jan-Jun;13(1):70-72. doi: 10.4103/ajts.AJTS_125_17.
Direct Antiglobulin Test is a method of demonstrating the presence of antibody/ complement bound to red cell membrane by using AHG to form a visible agglutination reaction. DAT positivity is seen in immune mediated haemolytic anaemias, however rarely non immune mediate haemolytic anaemias also show DAT positivity. DAT positivity predictive of 83% of autoimmune haemolytic anaemia and 1.4% cases without haemolytic anaemia. Screening of blood donors for DAT is usually not recommended traditionally by any guidelines. However DAT positivity is reported in 0.008% of donors. On extensive search of literature we could find only very few studies on DAT positivity in donors. We report two cases of DAT positive donors with no clinical or laboratory evidence of hemolysis.
直接抗球蛋白试验是一种通过使用抗人球蛋白（AHG）形成可见的凝集反应来证明抗体/补体与红细胞膜结合存在的方法。直接抗球蛋白试验阳性可见于免疫介导的溶血性贫血，然而很少有非免疫介导的溶血性贫血也表现为直接抗球蛋白试验阳性。直接抗球蛋白试验阳性预测83%的自身免疫性溶血性贫血和1.4%无溶血性贫血的病例。传统上，任何指南通常都不建议对献血者进行直接抗球蛋白试验筛查。然而，有0.008%的献血者报告直接抗球蛋白试验阳性。在广泛查阅文献时，我们仅能找到极少数关于献血者直接抗球蛋白试验阳性的研究。我们报告了两例直接抗球蛋白试验阳性的献血者，他们没有溶血的临床或实验室证据。
