Wu De-Hong, Xu Li, Xie Guan-Qun, Fan Yong-Sheng, Zhou Jia
The Second Affiliated Hospital of Zhejiang Chinese Medical University, 318 Chaowang Road, Hangzhou 310005, Zhejiang, China.
College of Basic Medical Sciences, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou 310053, Zhejiang, China.
Evid Based Complement Alternat Med. 2019 Jul 9;2019:9824906. doi: 10.1155/2019/9824906. eCollection 2019.
Heat syndrome is a folk saying in China, which is used to describe people with symptoms such as aphtha, oral ulcer, glossitis, swelling and aching of gingiva, and dry eye. Debx. (A), Rosc. (Z), and Presl (C) are the representatives of pungent and hot Chinese herbs which may cause heat syndrome. In order to explore the mechanism of pungent herbs-induced heat syndrome, rats were treated with AZC extracts at different concentrations and at different time periods. A series of cytokines were determined using the cytokine antibody array; some immunosuppressive cytokines, including TGF-, IL-10, and IL-35, significantly increased in AZC group as compared with control group. Higher mRNA expressions of Foxp3, TGF-, IL-10, and IL-35 were found in the spleen and thymus of rats after treatment for 18 days based on RT-PCR. Flow cytometry result revealed that the percentage of CD4CD25 Treg cells and Foxp3CD4CD25 Treg cells in spleen lymphocytes showed an increasing trend from the 3 day to the 18 day after treatment with middle dose of AZC extracts. It is speculated that extracts of AZC herbs may affect the development of heat syndrome by influencing Treg cells and immunosuppressive cytokines.
热证是中国的一种民间说法，用于描述患有诸如口疮、口腔溃疡、舌炎、牙龈肿痛和眼睛干涩等症状的人。荜茇（A）、玫瑰（Z）和水菖蒲（C）是可能导致热证的辛辣温热中药的代表。为了探究辛辣中药致热证的机制，将大鼠用不同浓度和不同时间段的荜茇-水菖蒲-玫瑰提取物进行处理。使用细胞因子抗体芯片测定一系列细胞因子；与对照组相比，荜茇-水菖蒲-玫瑰组中一些免疫抑制细胞因子，包括转化生长因子-β、白细胞介素-10和白细胞介素-35显著增加。基于逆转录-聚合酶链反应，在处理18天后发现大鼠脾脏和胸腺中叉头框蛋白3、转化生长因子-β、白细胞介素-10和白细胞介素-35的信使核糖核酸表达较高。流式细胞术结果显示，用中剂量荜茇-水菖蒲-玫瑰提取物处理后，从第3天到第18天，脾脏淋巴细胞中CD4⁺CD25⁺调节性T细胞和叉头框蛋白3⁺CD4⁺CD25⁺调节性T细胞的百分比呈上升趋势。推测荜茇-水菖蒲-玫瑰中药提取物可能通过影响调节性T细胞和免疫抑制细胞因子来影响热证的发生发展。
