Wang Zhihong, Long Ruiqing, Peng Mijun, Li Te, Shi Shuyun
Guangdong Provincial Public Laboratory of Analysis and Testing Technology, Guangdong Institute of Analysis, Guangzhou 510070, China.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
J Anal Methods Chem. 2019 Jul 8;2019:1505878. doi: 10.1155/2019/1505878. eCollection 2019.
Ferulic acid (FA), an important phenolic acid, is widely distributed in higher plants and presents many pharmacological effects. Therefore, sensitive determination of FA in complex matrix is necessary. Molecularly imprinted polymers-coated CdTe quantum dots (CdTe-QDs@MIPs) exhibited incomparable advantages because of their combination of excellent selectivity of MIPs and high sensitivity of QDs. Here, a fluorescent probe based on CdTe-QDs@MIPs was successfully fabricated for selective and sensitive determination of FA. MIPs shell was obtained by the reverse microemulsion method using FA, 3-(aminopropyl) triethoxysilane (APTES), and tetraethyl orthosilicate (TEOS), as template, functional monomer, and crosslinker. In optimal conditions, the fluorescence CdTe-QDs@MIPs sensor exhibited fast response (within only 3 min), high sensitivity (limit of detection, LOD at 0.85 g/l), excellent linear ranges (2-100 g/l) with a correlation coefficient of 0.9996, and distinguished selectivity for FA. Satisfactory recoveries from 91.8% to 110.3% were achieved with precisions below 6.6% for FA analysis in real pineapple juice and apple juice by developed CdTe-QDs@MIPs. The fluorescence results coincided well with those obtained by high-performance liquid chromatography (HPLC). It could be concluded that the resultant CdTe-QDs@MIPs offered a new way for rapid and sensitive analysis of FA in the complex matrix.
阿魏酸（FA）是一种重要的酚酸，广泛分布于高等植物中，并具有多种药理作用。因此，在复杂基质中灵敏测定FA是必要的。分子印迹聚合物包覆的碲化镉量子点（CdTe-QDs@MIPs）因其兼具MIPs的优异选择性和QDs的高灵敏度而展现出无与伦比的优势。在此，成功制备了一种基于CdTe-QDs@MIPs的荧光探针，用于选择性灵敏测定FA。以FA、3-（氨丙基）三乙氧基硅烷（APTES）和正硅酸四乙酯（TEOS）作为模板、功能单体和交联剂，通过反向微乳液法获得MIPs壳层。在最佳条件下，荧光CdTe-QDs@MIPs传感器表现出快速响应（仅3分钟内）、高灵敏度（检测限，LOD为0.85μg/l）、出色的线性范围（2-100μg/l），相关系数为0.9996，以及对FA具有显著的选择性。通过所制备的CdTe-QDs@MIPs对实际菠萝汁和苹果汁中的FA进行分析，回收率令人满意，在91.8%至110.3%之间，精密度低于6.6%。荧光结果与高效液相色谱（HPLC）所得结果吻合良好。可以得出结论，所得的CdTe-QDs@MIPs为复杂基质中FA的快速灵敏分析提供了一种新方法。
