Domínguez-Borbor Cristóbal, Betancourt Irma, Panchana Fanny, Sonnenholzner Stanislaus, Bayot Bonny
Escuela Superior Politécnica del Litoral, ESPOL, Centro Nacional de Acuicultura e Investigaciones Marinas, CENAIM, Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863, Guayaquil, Ecuador.
Escuela Superior Politécnica del Litoral, ESPOL, Facultad de Ingeniería Marítima y Ciencias del Mar, FIMCM, Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863, Guayaquil, Ecuador.
MethodsX. 2019 Jul 10;6:1617-1626. doi: 10.1016/j.mex.2019.07.007. eCollection 2019.
White spot syndrome virus (WSSV) is one of the most virulent pathogens of cultured penaeid shrimp. Several control strategies are used commonly to mitigate the economic losses caused by the pathogen, such as application of antiviral products at farm level. One of the most practical method for the screening of potential anti-WSSV products is through challenge tests. Therefore, it is essential to develop simple, reproducible and effective bioassays able to simulate specific mortality levels. The purpose of this study was to develop a simple and reproducible bioassay that simulate different mortality levels by varying the proportion of WSSV-infected and noninfected shrimp tissues administered to susceptible shrimp during a challenge test. This method mimics one of the natural transmission routes of WSSV infection in shrimp and could be applied to identify potential antiviral products to different cultured shrimp species susceptible to WSSV. Here we report: •A simple and economic method to evaluate therapeutic antiviral products against WSSV through a challenge test, that uses different biomass amounts of WSSV-infected papilla.•Allows to simulate a wide and reproducible range of mortalities observed in shrimp farms.•A challenge test that simulates one mode of natural WSSV infection in shrimp.
白斑综合征病毒（WSSV）是养殖对虾中最具致病性的病原体之一。人们通常采用几种控制策略来减轻该病原体造成的经济损失，例如在养殖场层面使用抗病毒产品。筛选潜在抗WSSV产品最实用的方法之一是通过攻毒试验。因此，开发能够模拟特定死亡率水平的简单、可重复且有效的生物测定方法至关重要。本研究的目的是开发一种简单且可重复的生物测定方法，通过在攻毒试验中改变给予易感对虾的感染WSSV和未感染WSSV的对虾组织比例来模拟不同的死亡率水平。该方法模拟了WSSV在对虾中自然传播的途径之一，可用于鉴定对不同易感染WSSV的养殖对虾品种有潜在抗病毒作用的产品。在此我们报告：•一种通过攻毒试验评估针对WSSV的治疗性抗病毒产品的简单且经济的方法，该方法使用不同生物量的感染WSSV的触角。•能够模拟在对虾养殖场观察到的广泛且可重复的死亡率范围。•一种模拟WSSV在对虾中自然感染模式的攻毒试验。
