Burckhardt J, Seelig R, Renger F, Calvo-Riera F, Liehr H, Seelig H P
Institut für Immunologie und Experimentelle Pathologie GmbH, Karlsruhe.
Immun Infekt. 1988 Jun;16(3):97-9.
An anti-D-immunoglobulin preparation implicated in a hepatitis non-A,non-B transmission was analyzed for the presence of a DNA, which was originally isolated, cloned and sequenced from feces of a patient with posttransfusion HNANB. The investigation was performed by a DNA polymerase chain reaction using synthetic oligoprimers. Commercially available immunoglobulin preparations served as controls. The demonstration of identical DNA sequences in the infectious material speaks in favour of this up to now unknown circular and partially double-stranded DNA to be a virus genome involved in hepatitis non-A,non-B.
对一种与非甲非乙型肝炎传播有关的抗-D免疫球蛋白制剂进行了分析，以检测是否存在一种DNA，该DNA最初是从一名输血后非甲非乙型肝炎患者的粪便中分离、克隆和测序得到的。调查是通过使用合成寡核苷酸引物的DNA聚合酶链反应进行的。市售免疫球蛋白制剂用作对照。在感染性物质中发现相同的DNA序列表明，这种迄今为止未知的环状和部分双链DNA可能是参与非甲非乙型肝炎的病毒基因组。
