Institute for Materials Science and Max Bergmann Center of Biomaterials , Hallwachsstraße 3 , 01062 Dresden , Germany.
Ruđer Bošković Institute , Bijenička cesta 54 , 10000 Zagreb , Croatia.
Langmuir. 2019 Aug 27;35(34):11114-11122. doi: 10.1021/acs.langmuir.9b01830. Epub 2019 Aug 8.
Peptides are very common recognition entities that are usually attached to surfaces using multistep processes. These processes require modification of the native peptides and of the substrates. Using functional groups in native peptides for their assembly on surfaces without affecting their biological activity can facilitate the preparation of biosensors. Herein, we present a simple single-step formation of native oxytocin monolayer on gold surface. These surfaces were characterized by atomic force spectroscopy, spectroscopic ellipsometry, and X-ray photoelectron spectroscopy. We took advantage of the native disulfide bridge of the oxytocin for anchoring the peptide to the Au surface, while preserving the metal-ion binding properties. Self-assembled oxytocin monolayer was used by electrochemical impedance spectroscopy for metal-ion sensing leading to subnanomolar sensitivities for zinc or copper ions.
肽是非常常见的识别实体，通常使用多步过程附着在表面上。这些过程需要对天然肽和底物进行修饰。在不影响其生物活性的情况下，使用天然肽中的官能团在表面上进行组装，可以方便生物传感器的制备。在此，我们提出了一种在金表面上简单的一步形成天然催产素单层的方法。这些表面通过原子力光谱学、光谱椭圆光谱学和 X 射线光电子能谱进行了表征。我们利用催产素的天然二硫键将肽固定在 Au 表面上，同时保留其金属离子结合特性。自组装的催产素单层通过电化学阻抗谱用于金属离子传感，对锌或铜离子具有亚纳摩尔的灵敏度。
