Institute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem, 9190401, Israel.
Indian Institute of Science Education and Research, Pune, 411008, India.
Chemistry. 2022 Oct 4;28(55):e202202193. doi: 10.1002/chem.202202193. Epub 2022 Aug 18.
Heparan sulfate glycosaminoglycans provides extracellular matrix defense against heavy metals cytotoxicity. Identifying the precise glycan sequences that bind a particular heavy metal ion is a key for understanding those interactions. Here, electrochemical and surface characterization techniques were used to elucidate the relation between the glycans structural motifs, uronic acid stereochemistry, and sulfation regiochemistry to heavy metal ions binding. A divergent strategy was employed to access a small library of structurally well-defined tetrasaccharides analogs with different sulfation patterns and uronic acid compositions. These tetrasaccharides were electrochemically grafted onto glassy carbon electrodes and their response to heavy metal ions was monitored by electrochemical impedance spectroscopy. Key differences in the binding of Hg(II), Cd(II), and Pb(II) were associated with a combination of the uronic acid type and the sulfation pattern.
硫酸乙酰肝素糖胺聚糖为细胞外基质提供重金属细胞毒性防御。确定与特定重金属离子结合的精确聚糖序列是理解这些相互作用的关键。在这里，电化学和表面表征技术被用于阐明聚糖结构基序、糖醛酸立体化学和硫酸化区域化学与重金属离子结合的关系。采用发散策略来获得具有不同硫酸化模式和糖醛酸组成的结构明确的四糖类似物的小文库。这些四糖通过电化学接枝到玻碳电极上，并通过电化学阻抗谱监测它们对重金属离子的响应。Hg(II)、Cd(II)和 Pb(II)的结合存在显著差异，这与糖醛酸类型和硫酸化模式的组合有关。
