Westerterp K R, Brouns F, Saris W H, ten Hoor F
Department of Human Biology, University of Limburg, Maastricht, The Netherlands.
J Appl Physiol (1985). 1988 Jul;65(1):53-6. doi: 10.1152/jappl.1988.65.1.53.
In previous studies the doubly labeled water method for measuring energy expenditure in free-living humans has been validated against respirometry under sedentary conditions. In the present investigation, energy expenditure is measured simultaneously with doubly labeled water and respirometry at low- and high-activity levels. Over 6 days, five subjects were measured doing mainly sedentary activities like desk work; their average daily metabolic rate was 1.40 +/- 0.09 (SD) times sleeping metabolic rate. Four subjects were measured twice over 3.5 days, including 2 days with heavy bicycle ergometer work, resulting in an average daily metabolic rate of 2.61 +/- 0.25 (SD) times sleeping metabolic rate. At the low-activity level, energy expenditures from the doubly labeled water method were on the average 1.4 +/- 3.9% (SD) larger than those from respirometry. At the high-activity level, the doubly labeled water method yielded values that were 1.0 +/- 7.0% (SD) lower than those from respirometry. Results demonstrate the utility of the doubly labeled water method for the determination of energy expenditure in the range of activity levels in daily life.
在以往的研究中，用于测量自由活动人群能量消耗的双标水法已在久坐条件下通过与呼吸测定法对比得到验证。在本研究中，在低活动水平和高活动水平下同时使用双标水法和呼吸测定法测量能量消耗。在6天时间里，对5名主要进行久坐活动（如伏案工作）的受试者进行了测量；他们的平均每日代谢率是睡眠代谢率的1.40±0.09（标准差）倍。对4名受试者在3.5天内进行了两次测量，其中包括2天繁重的自行车测力计运动，其平均每日代谢率为睡眠代谢率的2.61±0.25（标准差）倍。在低活动水平下，双标水法测得的能量消耗平均比呼吸测定法测得的能量消耗大1.4±3.9%（标准差）。在高活动水平下，双标水法得出的值比呼吸测定法测得的值低1.0±7.0%（标准差）。结果表明双标水法在测定日常生活活动水平范围内的能量消耗方面具有实用性。
