Department of Chemistry and Chemical Biology , Rutgers University , Piscataway , New Jersey 08854 , United States.
Department of Chemical and Biomolecular Engineering , Sogang University , Seoul 04107 , Republic of Korea.
ACS Nano. 2019 Aug 27;13(8):8793-8803. doi: 10.1021/acsnano.9b01875. Epub 2019 Aug 2.
The full realization of stem cell-based treatments for neurodegenerative diseases requires precise control and characterization of stem cell fate. Herein, we report a multifunctional magneto-plasmonic nanorod (NR)-based detection platform to address the limitations associated with the current destructive characterization methods of stem cell neurogenesis. Exosomes and their inner contents have been discovered to play critical roles in cell-cell interactions and intrinsic cellular regulations and have received wide attention as next-generation biomarkers. Moreover, exosomal microRNAs (miRNA) also offer an essential avenue for nondestructive molecular analyses of cell cytoplasm components. To this end, our developed nondestructive, selective, and sensitive detection platform has (i) an immunomagnetic active component for exosome isolation and (ii) a plasmonic/metal-enhanced fluorescence component for sensitive exosomal miRNA detection to characterize stem cell differentiation. In a proof-of-concept demonstration, our multifunctional magneto-plasmonic NR successfully detected the expression level of miRNA-124 and characterized neurogenesis of human-induced pluripotent stem cell-derived neural stem cells in a nondestructive and efficient manner. Furthermore, we demonstrated the versatility and feasibility of our multifunctional magneto-plasmonic NRs by characterizing a heterogeneous population of neural cells in an rodent model. Collectively, we believe our multifunctional magneto-plasmonic NR-based exosomal miRNA detection platform has a great potential to investigate the function of cell-cell interactions and intrinsic cellular regulators for controlling stem cell differentiation.
为了实现基于干细胞的神经退行性疾病治疗，需要精确控制和描述干细胞的命运。在此，我们报告了一种多功能磁等离子纳米棒（NR）基检测平台，以解决当前干细胞神经发生破坏性特征分析方法的局限性。现已发现外泌体及其内部内容物在细胞间相互作用和内在细胞调节中起着关键作用，并作为下一代生物标志物受到广泛关注。此外，外泌体 microRNA（miRNA）也为细胞质成分的非破坏性分子分析提供了重要途径。为此，我们开发的非破坏性、选择性和灵敏的检测平台具有：（i）用于外泌体分离的免疫磁活性成分，以及（ii）用于灵敏外泌体 miRNA 检测的等离子体/金属增强荧光成分，以对干细胞分化进行特征描述。在概念验证演示中，我们的多功能磁等离子 NR 成功检测了 miRNA-124 的表达水平，并以非破坏性和高效的方式对人诱导多能干细胞衍生的神经干细胞的神经发生进行了特征描述。此外，我们通过对啮齿动物模型中的异质神经细胞群进行特征描述，证明了我们的多功能磁等离子 NR 的多功能性和可行性。总之，我们相信我们基于多功能磁等离子 NR 的外泌体 miRNA 检测平台有很大的潜力来研究细胞间相互作用和内在细胞调节的功能，以控制干细胞分化。
