Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy , Jinan University , Guangzhou 510632 , People's Republic of China.
Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research , Jinan University , Guangzhou 510632 , People's Republic of China.
J Nat Prod. 2019 Aug 23;82(8):2349-2353. doi: 10.1021/acs.jnatprod.9b00322. Epub 2019 Jul 30.
Three new linear peptides containing d-leucine, named whitmantides A-C (-), were isolated from the dried whole bodies of Whitman. Their structures with absolute configurations were elucidated by Edman degradation, mass spectrometry, Marfey's analysis, and solid-phase synthesis. It is the first time that peptides containing d-amino acid in leeches were discovered. Compounds - displayed neuroprotective activities against oxygen-glucose deprivation/reperfusion injury on Neuro-2a cells. In addition, ex vivo serum stability tests showed that - were resistant to protease degradation.
从Whitman 的干整体中分离出三个含有 d-亮氨酸的新线性肽，命名为Whitmantides A-C(-)。通过 Edman 降解、质谱、Marfey 分析和固相合成阐明了它们的结构和绝对构型。这是首次在水蛭中发现含有 d-氨基酸的肽。化合物-对Neuro-2a 细胞的氧葡萄糖剥夺/再灌注损伤具有神经保护活性。此外，体外血清稳定性测试表明-能够抵抗蛋白酶的降解。
