Smith Adam N, Märker Katharina, Hediger Sabine, De Paëpe Gaël
Univ. Grenoble Alpes, CEA, CNRS, IRIG, MEM , F-38000 Grenoble , France.
J Phys Chem Lett. 2019 Aug 15;10(16):4652-4662. doi: 10.1021/acs.jpclett.8b03874. Epub 2019 Aug 2.
Dynamic nuclear polarization (DNP) has made feasible solid-state NMR experiments that were previously thought impractical due to sensitivity limitations. One such class of experiments is the structural characterization of organic and biological samples at natural isotopic abundance (NA). Herein, we describe the many advantages of DNP-enabled ssNMR at NA, including the extraction of long-range distance constraints using dipolar recoupling pulse sequences without the deleterious effects of dipolar truncation. In addition to the theoretical underpinnings in the analysis of these types of experiments, numerous applications of DNP-enabled ssNMR at NA are discussed.
动态核极化（DNP）使以前由于灵敏度限制而被认为不切实际的固态核磁共振实验成为可能。这类实验中的一个例子是在天然同位素丰度（NA）下对有机和生物样品进行结构表征。在此，我们描述了在NA条件下利用DNP的固态核磁共振的诸多优势，包括使用偶极重耦合脉冲序列提取长程距离约束，而不会受到偶极截断的有害影响。除了对这类实验分析的理论基础外，还讨论了在NA条件下利用DNP的固态核磁共振的众多应用。
