Department of Chemistry, Michigan State University, East Lansing, MI 48824, USA.
Department of Chemistry, Michigan State University, East Lansing, MI 48824, USA.
Structure. 2023 Nov 2;31(11):1375-1385. doi: 10.1016/j.str.2023.07.012. Epub 2023 Aug 18.
Structural analysis of macromolecular complexes within their natural cellular environment presents a significant challenge. Recent applications of solid-state NMR (ssNMR) techniques on living fungal cells and intact plant tissues have greatly enhanced our understanding of the structure of extracellular matrices. Here, we selectively highlight the most recent progress in this field. Specifically, we discuss how ssNMR can provide detailed insights into the chemical composition and conformational structure of pectin, and the consequential impact on polysaccharide interactions and cell wall organization. We elaborate on the use of ssNMR data to uncover the arrangement of the lignin-polysaccharide interface and the macrofibrillar structure in native plant stems or during degradation processes. We also comprehend the dynamic structure of fungal cell walls under various morphotypes and stress conditions. Finally, we assess how the combination of NMR with other techniques can enhance our capacity to address unresolved structural questions concerning these complex macromolecular assemblies.
在天然细胞环境中对大分子复合物进行结构分析是一项重大挑战。近年来，固态 NMR（ssNMR）技术在活真菌细胞和完整植物组织上的应用极大地增进了我们对细胞外基质结构的理解。在此，我们特别强调该领域的最新进展。具体而言，我们讨论了 ssNMR 如何提供有关果胶的化学组成和构象结构的详细信息，以及对多糖相互作用和细胞壁组织的影响。我们详细介绍了使用 ssNMR 数据揭示天然植物茎中的木质素-多糖界面以及宏观纤维结构的排列，以及在降解过程中的排列。我们还理解了在各种形态和应激条件下真菌细胞壁的动态结构。最后，我们评估了将 NMR 与其他技术结合使用如何增强我们解决这些复杂大分子组装体中未解决结构问题的能力。
