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固态 NMR 研究藻类、细菌、真菌和植物的细胞外基质和细胞壁。

Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants.

机构信息

Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, United States.

Department of Chemistry, Université du Québec à Montréal, Montreal H2X 2J6, Canada.

出版信息

Chem Rev. 2022 May 25;122(10):10036-10086. doi: 10.1021/acs.chemrev.1c00669. Epub 2021 Dec 8.

DOI:10.1021/acs.chemrev.1c00669
PMID:34878762
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9486976/
Abstract

Extracellular matrixes (ECMs), such as the cell walls and biofilms, are important for supporting cell integrity and function and regulating intercellular communication. These biomaterials are also of significant interest to the production of biofuels and the development of antimicrobial treatment. Solid-state nuclear magnetic resonance (ssNMR) and magic-angle spinning-dynamic nuclear polarization (MAS-DNP) are uniquely powerful for understanding the conformational structure, dynamical characteristics, and supramolecular assemblies of carbohydrates and other biomolecules in ECMs. This review highlights the recent high-resolution investigations of intact ECMs and native cells in many organisms spanning across plants, bacteria, fungi, and algae. We spotlight the structural principles identified in ECMs, discuss the current technical limitation and underexplored biochemical topics, and point out the promising opportunities enabled by the recent advances of the rapidly evolving ssNMR technology.

摘要

细胞外基质（ECMs），如细胞壁和生物膜，对于支持细胞完整性和功能以及调节细胞间通讯非常重要。这些生物材料对于生物燃料的生产和抗菌处理的发展也具有重要意义。固态核磁共振（ssNMR）和魔角旋转-动态核极化（MAS-DNP）对于理解 ECM 中碳水化合物和其他生物分子的构象结构、动力学特性和超分子组装具有独特的强大作用。本综述重点介绍了最近在跨越植物、细菌、真菌和藻类等多种生物体的完整 ECM 和天然细胞中的高分辨率研究。我们突出了 ECM 中确定的结构原则，讨论了当前技术限制和未充分探索的生化主题，并指出了快速发展的 ssNMR 技术的最新进展所带来的有前途的机会。

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