Medical Research and Laboratory Diagnostic Center, Jinan Central Hospital Affiliated to Shandong University, Jinan, P.R. China.
DNA Cell Biol. 2019 Sep;38(9):962-968. doi: 10.1089/dna.2019.4647. Epub 2019 Jul 30.
Previous studies revealed that the rs671 polymorphism in the acetaldehyde dehydrogenase 2 () genes is correlated with alcohol consumption in Japanese population. The gene variants and drinking are associated with hypertension and dyslipidemia. However, it remains unclear whether there might be potent relationships among rs671 polymorphism, alcohol consumption, hypertension, and dyslipidemia in Shandong population. A total of 467 male volunteers from Shandong area were enrolled in this study. The rs671 polymorphism was genotyped using polymerase chain reaction-restriction fragment length polymorphism. The concentrations of total cholesterol (TC), triglycerides, low-density lipoprotein, and high-density lipoprotein (HDL) in serum were measured using commercial kits. SPSS 23.0 was used for statistical analysis. The significance of differences between subgroups was determined using chi-square test, and multiple comparisons were performed with the least-significant difference method. The variant frequencies were 80.5% with GG, 17.1% with GA, and 2.4% with AA. The genotypes had significant correlations with alcohol consumption ( = 0.001), whereas the GA genotype was associated with a decreased risk of alcohol consumption (odds ratio = 0.27; 95% confidence interval = 0.130-0.539; = 0.001). The genotypes frequencies and drinking habits were significantly different between hypertension and healthy individuals ( = 0.034; = 0.044). The GG genotype individuals have high average lipids levels, and the proportion of TC disorder among GG individuals was higher than that of GA individuals ( = 0.006). Individuals who had drinking habits have a high average lipids levels; especially average TC levels ( = 0.048), and had high proportions of dyslipidemia (TC and HDL; = 0.016 and = 0.033, respectively). The frequencies of variants were evaluated according to the Hardy-Weinberg equilibrium among enrolled population. Our study suggested that the individuals with rs671 GA genotype were less prone to developing a drinking habit in Shandong population. The genotypes and drinking habit were associated with hypertension and lipid profiles especially TC profile in Shandong province. The rs671 genotypes indicated that the gene-related drinking habit and gene variant altogether may affect hypertension and dyslipidemia.
先前的研究表明，乙醛脱氢酶 2（）基因中的 rs671 多态性与日本人群的饮酒量有关。基因变异与饮酒均与高血压和血脂异常相关。然而，在山东人群中，rs671 多态性、饮酒、高血压和血脂异常之间是否存在潜在的关联尚不清楚。本研究共纳入了 467 名来自山东地区的男性志愿者。采用聚合酶链反应-限制性片段长度多态性方法检测 rs671 多态性。采用商业试剂盒检测血清总胆固醇（TC）、甘油三酯、低密度脂蛋白和高密度脂蛋白（HDL）的浓度。采用 SPSS 23.0 进行统计分析。采用卡方检验比较亚组间的差异，采用最小显著差法进行多重比较。结果显示，rs671 变异频率分别为 80.5%（GG 基因型）、17.1%（GA 基因型）和 2.4%（AA 基因型）。基因型与饮酒量显著相关（=0.001），GA 基因型与饮酒量减少相关（比值比=0.27；95%置信区间=0.130-0.539；=0.001）。高血压患者与健康个体的基因型频率和饮酒习惯存在显著差异（=0.034；=0.044）。GG 基因型个体的血脂水平较高，且 GG 个体 TC 异常比例高于 GA 个体（=0.006）。有饮酒习惯的个体血脂水平较高，尤其是 TC 水平（=0.048），且血脂异常比例较高（TC 和 HDL；=0.016 和=0.033）。根据纳入人群的 Hardy-Weinberg 平衡评估 rs671 变异的频率。本研究表明，在山东人群中，rs671 GA 基因型个体饮酒的可能性较小。基因型和饮酒习惯与高血压和血脂谱有关，尤其是 TC 谱。rs671 基因型表明，基因相关的饮酒习惯和基因变异可能共同影响高血压和血脂异常。
