Laboratory of Molecular Genetics, Kazan State Medical University, Kazan, Russia.
Faculty of Medicine, University of Information Technology and Management in Rzeszow, Poland.
J Strength Cond Res. 2019 Oct;33(10):2602-2607. doi: 10.1519/JSC.0000000000003304.
Grishina, EE, Zmijewski, P, Semenova, EA, Cięszczyk, P, Humińska-Lisowska, K, Michałowska-Sawczyn, M, Maculewicz, E, Crewther, B, Orysiak, J, Kostryukova, ES, Kulemin, NA, Borisov, OV, Khabibova, SA, Larin, AK, Pavlenko, AV, Lyubaeva, EV, Popov, DV, Lysenko, EA, Vepkhvadze, TF, Lednev, EM, Bondareva, EA, Erskine, RM, Generozov, EV, and Ahmetov, II. Three DNA polymorphisms previously identified as markers for handgrip strength are associated with strength in weightlifters and muscle fiber hypertrophy. J Strength Cond Res 33(10): 2602-2607, 2019-Muscle strength is a highly heritable trait. So far, 196 single nucleotide polymorphisms (SNPs) associated with handgrip strength have been identified in 3 genome-wide association studies. The aim of our study was to validate the association of 35 SNPs with strength of elite Russian weightlifters and replicate the study in Polish weightlifters. Genotyping was performed using micro-array analysis or real-time polymerase chain reaction. We found that the rs12055409 G-allele near the MLN gene (p = 0.004), the rs4626333 G-allele near the ZNF608 gene (p = 0.0338), and the rs2273555 A-allele in the GBF1 gene (p = 0.0099) were associated with greater competition results (total lifts in snatch and clean and jerk adjusted for sex and weight) in 53 elite Russian weightlifters. In the replication study of 76 sub-elite Polish weightlifters, rs4626333 GG homozygotes demonstrated greater competition results (p = 0.0155) and relative muscle mass (p = 0.046), adjusted for sex, weight, and age, compared with carriers of the A-allele. In the following studies, we tested the hypotheses that these SNPs would be associated with skeletal muscle hypertrophy and handgrip strength. We found that the number of strength-associated alleles was positively associated with fast-twitch muscle fiber cross-sectional area in the independent cohort of 20 male power athletes (p = 0.021) and with handgrip strength in 87 physically active individuals (p = 0.015). In conclusion, by replicating previous findings in 4 independent studies, we demonstrate that the rs12055409 G-, rs4626333 G-, and rs2273555 A-alleles are associated with higher levels of strength, muscle mass, and muscle fiber size.
格里希娜，EE，兹米亚京斯基，P，塞米诺娃，EA，采切克，P，胡米娜-利索娃，K，米哈洛夫斯卡-萨温琴，M，马库列维奇，E，克鲁瑟，B，奥里西阿克，J，科斯特鲁科娃，ES，库里明，NA，鲍里索夫，OV，哈比博娃，SA，拉林，AK，帕夫连科，AV，柳巴耶娃，EV，波波夫，DV，列先科，EA，韦普赫瓦德泽，TF，列德涅夫，EM，邦达列娃，EA，埃尔斯金，RM，杰尼索夫，EV，和阿赫梅托夫，II。 以前确定为握力标志物的 3 个 DNA 多态性与举重运动员的力量和肌肉纤维肥大有关。J 力量与适应研究 33(10)：2602-2607，2019-肌肉力量是一种高度可遗传的特征。到目前为止，已经在 3 项全基因组关联研究中确定了 196 个与握力相关的单核苷酸多态性(SNP)。我们研究的目的是验证 35 个 SNP 与俄罗斯精英举重运动员力量的相关性，并在波兰举重运动员中复制该研究。基因分型使用微阵列分析或实时聚合酶链反应进行。我们发现，位于 MLN 基因附近的 rs12055409 G-等位基因(p = 0.004)、位于 ZNF608 基因附近的 rs4626333 G-等位基因(p = 0.0338)和位于 GBF1 基因中的 rs2273555 A-等位基因(p = 0.0099)与 53 名俄罗斯精英举重运动员的比赛成绩(经性别和体重调整的抓举和挺举总成绩)相关。在对 76 名次精英波兰举重运动员的复制研究中，与携带 A-等位基因的运动员相比，rs4626333 GG 纯合子表现出更大的比赛成绩(p = 0.0155)和相对肌肉质量(p = 0.046)，经性别、体重和年龄调整。在接下来的研究中，我们测试了这些 SNP 与骨骼肌肥大和握力相关的假设。我们发现，在独立的 20 名男性力量运动员队列中，与力量相关的等位基因数量与快肌纤维横截面积呈正相关(p = 0.021)，与 87 名活跃个体的握力呈正相关(p = 0.015)。总之，通过在 4 项独立研究中复制先前的发现，我们证明 rs12055409 G-、rs4626333 G-和 rs2273555 A-等位基因与更高的力量、肌肉质量和肌肉纤维大小水平相关。
