Department of Pharmaceutical Engineering, Soonchunhyang University, Asan, Republic of Korea.
Nanotechnology. 2019 Nov 1;30(44):445502. doi: 10.1088/1361-6528/ab36c9.
We present a quartz crystal microbalance (QCM) immunosensor for highly sensitive detection of prostate-specific antigen (PSA) in a human serum immunoassay. In particular, in this study, we employed signal amplification using and enlarging gold nanoparticles. Because QCM measures the change of resonance frequency according to the mass change occurring on the sensor surface, we could quantitatively analyze PSA based on a tremendous increase in mass by sandwich immunoassay using AuNP-conjugated anti-PSA-detecting antibody enhanced with subsequent gold staining. The limit of detection of the PSA immunoassay in human serum without gold staining enhancement was 687 pg ml but was 48 pg ml with the gold staining-mediated signal amplification. That is, amplifying the signal resulted in increased sensitivity and reproducibility of immunoassay in a human serum.
我们提出了一种石英晶体微天平（QCM）免疫传感器，用于在人血清免疫分析中高度灵敏地检测前列腺特异性抗原（PSA）。特别地，在这项研究中，我们使用并放大了金纳米粒子进行信号放大。由于 QCM 根据传感器表面发生的质量变化测量共振频率的变化，因此我们可以通过使用 AuNP 缀合的抗 PSA 检测抗体进行三明治免疫分析，并随后进行金染色来定量分析 PSA，从而通过大量增加质量来进行定量分析。未经金染色增强的人血清中 PSA 免疫分析的检测限为 687pg/ml，但通过金染色介导的信号放大，检测限为 48pg/ml。也就是说，信号放大提高了人血清中免疫分析的灵敏度和重现性。
