Department of Pharmaceutical Engineering, Soonchunhyang University, Asan 31538, Republic of Korea.
Biosensors (Basel). 2024 Oct 11;14(10):494. doi: 10.3390/bios14100494.
In this paper, we present a sensitive and highly reproducible fluorescence immunosensor for detecting PSA in human serum. A unique feature of this study is that it uses creatively designed paddle screw-type devices and their custom-made rotating system for PSA immunoassay. The paddle screw devices were designed to maximize the surface-to-volume ratio over which the immunoassay reaction could occur to improve detection sensitivity. This paddle screw-based immunoassay offers an accessible and efficient method with a short analysis time of less than 30 min. Active rotation of the paddle screw plays a crucial role in fast and accurate analysis of PSA. Additionally, a paddle screw-based immunoassay and subsequent fluorescence detection using a custom prototype fluorescence detection system were compared to a typical well plate-based immunoassay system. Results of PSA detection in human serum showed that the detection sensitivity through the paddle screw-based analysis improved about five times compared to that with a well plate-based analysis.
本文提出了一种用于检测人血清中 PSA 的灵敏且重现性高的荧光免疫传感器。本研究的一个独特特点是创造性地设计了桨叶螺杆式装置及其定制的旋转系统，用于 PSA 免疫分析。桨叶螺杆装置的设计目的是最大限度地提高免疫反应发生的表面积与体积比，以提高检测灵敏度。这种基于桨叶螺杆的免疫分析提供了一种易于使用且高效的方法，分析时间不到 30 分钟。桨叶螺杆的主动旋转在 PSA 的快速准确分析中起着至关重要的作用。此外，还将基于桨叶螺杆的免疫分析以及随后使用定制原型荧光检测系统的荧光检测与典型的微孔板免疫分析系统进行了比较。人血清中 PSA 的检测结果表明，与基于微孔板的分析相比，基于桨叶螺杆的分析的检测灵敏度提高了约五倍。
