在 和 中进行水通道蛋白基因家族的全基因组分析及基因 的异源表达赋予其抗旱和耐盐性。
Gene-Wide Analysis of Aquaporin Gene Family in and Heterologous Expression of the Gene Confers Drought and Salinity Tolerance in .
机构信息
State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Life Sciences, Northwest A&F University, Yangling 712100, China.
State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, China.
出版信息
Int J Mol Sci. 2019 Jul 29;20(15):3710. doi: 10.3390/ijms20153710.
The aquaporins (AQPs) are a family of integral membrane proteins involved in the transcellular membrane transport of water and other small molecules. A scan of the apple () genome revealed the presence of 42 genes encoding putative AQPs. Based on a phylogenetic analysis of the deduced peptide sequences of the AQPs generated by , poplar (), and rubber (), the apple AQPs were each assigned membership of the five established AQP subfamilies, namely the PIPs (eleven members), the TIPs (thirteen members), the NIPs (eleven members), the SIPs (five members), and the XIPs (two members). The apple AQPs included asparagine-proline-alanine (NPA) motifs, an aromatic/arginine (ar/R) selectivity filter, and the Froger's positions. The heterologous expression of in was shown to enhance the level of tolerance exhibited against both drought and salinity.
水通道蛋白（AQPs）是一组参与跨细胞膜运输水和其他小分子的完整膜蛋白。对苹果基因组的扫描显示，存在 42 个编码假定 AQP 的基因。基于对通过推导的 AQP 肽序列的系统发育分析，杨树()和橡胶()，苹果 AQPs 分别被分配到五个已建立的 AQP 亚家族成员中，即 PIPs（十一个成员）、TIPs（十三个成员）、NIPs（十一个成员）、SIPs（五个成员）和 XIPs（两个成员）。苹果 AQPs 包括天冬酰胺-脯氨酸-丙氨酸（NPA）基序、芳香族/精氨酸（ar/R）选择性过滤器和 Froger 位置。在中异源表达 被证明可以提高对干旱和盐度的耐受水平。
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