Yamamoto Naoki, Masumura Takehiro, Yano Kentaro, Sugimoto Toshio
Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan.
Bioinformatics Laboratory, School of Agriculture, Meiji University, Kawasaki, Japan.
Biosci Biotechnol Biochem. 2019 Dec;83(12):2238-2243. doi: 10.1080/09168451.2019.1648205. Epub 2019 Jul 31.
The protein and oil contents in soybean seeds are major factors in seed quality. Seed proteins and oils are synthesized from sucrose and nitrogenous compounds transported into maturing seeds. In this study, we compared changes in the activity of phosphopyruvate carboxylase (PEPC) and the accumulation profiles of protein and oil in maturing seeds of two soybean cultivars, which exhibit different protein and oil contents in seeds, to determine the interrelationships of them. A principal component analysis indicated a concordance of seed PEPC activity with the protein content, but did not with the oil content. PEPC activity per seed was highest in the late maturation stage, when the physiological status of the vegetative organs drastically changed. The high-protein cultivar had higher PEPC activity compared to the low-protein cultivar. These results highlight the biological role of PEPC in the synthesis of protein, therefore it was implied that PEPC could be a biomarker in soybean breeding. ANOVA: analysis of variance; DS: developmental stage; DW: dry weight; FW: fresh weight; NIR: near infrared; PEP(C): phosphopyruvate (carboxylase); PC(A): principal component (analysis); S.E.: standard error; WC: water content.
大豆种子中的蛋白质和油含量是种子品质的主要因素。种子蛋白质和油由转运到成熟种子中的蔗糖和含氮化合物合成。在本研究中，我们比较了两个种子中蛋白质和油含量不同的大豆品种成熟种子中磷酸烯醇式丙酮酸羧化酶（PEPC）活性的变化以及蛋白质和油的积累情况，以确定它们之间的相互关系。主成分分析表明种子PEPC活性与蛋白质含量一致，但与油含量不一致。单个种子的PEPC活性在成熟后期最高，此时营养器官的生理状态发生了剧烈变化。高蛋白品种的PEPC活性高于低蛋白品种。这些结果突出了PEPC在蛋白质合成中的生物学作用，因此暗示PEPC可能成为大豆育种中的一个生物标志物。方差分析；发育阶段；干重；鲜重；近红外；磷酸烯醇式丙酮酸（羧化酶）；主成分（分析）；标准误差；含水量
