Piffaretti J C, Arini A, Frey J
Istituto Cantonale Batteriologico, Lugano, Switzerland.
Mol Gen Genet. 1988 May;212(2):215-8. doi: 10.1007/BF00334687.
The plasmid pUB307, a derivative of RP1, is a conjugative, broad-host-range plasmid. We have shown that this element mobilizes gonococcal resistance plasmids from Escherichia coli to Neisseria gonorrhoeae, thus providing evidence that extrachromosomal elements can efficiently enter gonococci by conjugation. Furthermore, pUB307 can also be used as a helper element to mobilize the cloning vector pLES2 into N. gonorrhoeae. This finding significantly increases the usefulness of pLES2 as a shuttle vector between E. coli and gonococcus.
质粒pUB307是RP1的衍生物，是一种可接合的广宿主范围质粒。我们已经证明，该元件可将淋病奈瑟菌耐药性质粒从大肠杆菌转移至淋病奈瑟菌，从而提供了染色体外元件可通过接合有效进入淋病奈瑟菌的证据。此外，pUB307还可作为辅助元件，将克隆载体pLES2转移至淋病奈瑟菌。这一发现显著提高了pLES2作为大肠杆菌和淋病奈瑟菌之间穿梭载体的实用性。
