Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, United Kingdom.
Department of Chemistry, University of Oxford, Oxford, United Kingdom.
Antimicrob Agents Chemother. 2018 Jul 27;62(8). doi: 10.1128/AAC.00130-18. Print 2018 Aug.
The tunicamycin biosynthetic gene cluster of consists of 14 genes ( to ) with a high degree of apparent translational coupling. Transcriptional analysis revealed that all of these genes are likely to be transcribed as a single operon from two promoters, p1 and p2. In-frame deletion analysis revealed that just six of these genes () are essential for tunicamycin production in the heterologous host , while five () with likely counterparts in primary metabolism are not necessary, but presumably ensure efficient production of the antibiotic at the onset of tunicamycin biosynthesis. Three genes are implicated in immunity, namely, and , which encode a two-component ABC transporter presumably required for export of the antibiotic, and , which encodes a putative -adenosylmethionine (SAM)-dependent methyltransferase. Expression of or in conferred resistance to exogenous tunicamycin. The results presented here provide new insights into tunicamycin biosynthesis and immunity.
衣霉素生物合成基因簇由 14 个基因（到）组成，具有高度明显的翻译偶联。转录分析表明，所有这些基因可能作为一个单一的操纵子从两个启动子 p1 和 p2 转录。框内缺失分析表明，只有这 6 个基因（）对于在异源宿主中的衣霉素生产是必需的，而五个（）与初级代谢中的对应物没有必要，但可能确保在衣霉素生物合成开始时有效生产抗生素。三个基因与免疫有关，即和，它们编码一个双组分 ABC 转运体，推测该转运体用于抗生素的外排，和，它编码一个假定的 -腺苷甲硫氨酸（SAM）依赖性甲基转移酶。在中表达或赋予对外源衣霉素的抗性。这里呈现的结果提供了对衣霉素生物合成和免疫的新见解。
