Ononamadu Chimaobi J, Alhassan Adamu J, Imam Abdullahi A, Ibrahim Aminu, Ihegboro Godwin O, Owolarafe Alowonle T, Sule Mohammed S
Department of Biochemistry and Forensic Science, Nigeria Police Academy, Wudil, Kano State, Nigeria.
Department of Biochemistry, Bayero University, Kano State, Nigeria.
Caspian J Intern Med. 2019 Spring;10(2):162-175. doi: 10.22088/cjim.10.2.162.
Diabetes is a metabolic disorder with a highly complex, multifaceted and intricate etiologies and thus may require management options that proffers multimodal mechanism of action. This present study evaluated the antidiabetic and antioxidant potential of the methanolic extract/fractions of leaves of
The antidiabetic potential was evaluated and using STZ-induced diabetic Wistar rat model () and inhibition of α-amylase and α-glucosidase activity (). Antioxidant activity was assessed by free radical scavenging and reducing power assays and via monitoring SOD and CAT activities; GSH and MDA levels.
The total phenolic content (221.0±3.0 mg catechol/g of sample) and tannins (146.0±4.0 mg tannic acid/g of sample) of the crude extract; and flavonoid of the aqueous-methanol fraction (216.0.0±1.0 mg of rutin/g of sample) were found to be significantly higher relative to others. The crude extract and the aqueous-methanol fraction exhibited a significantly (p<0.05) higher percentage reduction in fasting blood glucose and a concomitant increase in serum insulin level relative to the diabetic control group. The highest radical scavenging activity and reducing power were observed in the aqueous-methanol fraction. The aqueous-methanol solvent fraction also significantly reversed the alterations in oxidative stress markers occasioned by the diabetic condition.
In conclusion, the result of the present study has demonstrated evidently that extracts of leaves ameliorates hyperglycemia and the associated oxidative stress in STZ-induced rats.
糖尿病是一种代谢紊乱疾病，其病因高度复杂、多方面且错综复杂，因此可能需要具有多模式作用机制的治疗方案。本研究评估了[植物名称]叶片甲醇提取物/馏分的抗糖尿病和抗氧化潜力。
使用链脲佐菌素诱导的糖尿病Wistar大鼠模型评估抗糖尿病潜力，并检测α-淀粉酶和α-葡萄糖苷酶活性的抑制情况。通过自由基清除和还原能力测定评估抗氧化活性，并监测超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性、谷胱甘肽（GSH）和丙二醛（MDA）水平。
粗提物的总酚含量（221.0±3.0毫克儿茶酚/克样品）和单宁含量（146.0±4.0毫克单宁酸/克样品）；以及水-甲醇馏分的黄酮类化合物（216.0.0±1.0毫克芦丁/克样品）相对于其他成分显著更高。与糖尿病对照组相比，粗提物和水-甲醇馏分的空腹血糖降低百分比显著更高（p<0.05），同时血清胰岛素水平升高。水-甲醇馏分具有最高的自由基清除活性和还原能力。水-甲醇溶剂馏分还显著逆转了糖尿病状态引起的氧化应激标志物的变化。
总之，本研究结果清楚地表明，[植物名称]叶片提取物可改善链脲佐菌素诱导的大鼠的高血糖症及相关氧化应激。
