Department of Agriculture and Life Industry, Kangwon National University, Chuncheon, 24341, South Korea.
Department of Forest Environmental System, Kangwon National University, Chuncheon, 24341, South Korea.
Genes Genomics. 2019 Nov;41(11):1315-1327. doi: 10.1007/s13258-019-00846-2. Epub 2019 Jul 30.
Plant height is an important component of plant architecture and significantly affects crop breeding practices and yield. Dwarfism in plants prevents lodging and therefore it's a desired trait in crops.
To find differentially expressed genes to classify and understand the regulation of genes related to plant growth in mutant dwarf soybeans, which appeared in the F5 generation.
We obtained a few segregated dwarf soybeans in the populations derived from the crossing of Glycine max var. Peking and Glycine soja var. IT182936 in an F5 RIL population. These dwarf soybeans may be useful genetic resources for plant breeders, geneticists and biologists. Using the Illumina high-throughput platform, transcriptomes were generated and compared among normal and dwarf soybeans in triplicate.
We found complex relationship of the expressed genes to plant growth. There were highly significantly up-/downregulated genes according to the comparison of gene expression in normal and dwarf soybeans. The genes related to disease and stress responses were found to be upregulated in dwarf soybeans. Such over-expression of disease resistance and other immune response genes can be targeted to understand how the immune genes regulate the response of plant growth. In addition, photosynthesis-related genes showed very low expression in dwarf lines. The transcriptome expression and genes classified as related to plant growth may be useful resources to researchers studying plant growth.
株高是植物结构的一个重要组成部分，对作物的选育实践和产量有显著影响。植物的矮化可以防止倒伏，因此在作物中是一个理想的特性。
在 F5 代出现的突变矮化大豆中，寻找差异表达基因，对与植物生长相关的基因进行分类和调控。
我们从普通大豆（Glycine max var. Peking）和野生大豆（Glycine soja var. IT182936）杂交的 F5 RIL 群体中，获得了几个分离的矮化大豆。这些矮化大豆可能是植物育种家、遗传学家和生物学家有用的遗传资源。使用 Illumina 高通量平台，在三倍体中生成并比较了正常和矮化大豆之间的转录组。
我们发现表达基因与植物生长之间存在复杂的关系。根据正常和矮化大豆之间的基因表达比较，发现了高度显著的上调/下调基因。与疾病和应激反应相关的基因在矮化大豆中上调。这种对疾病抗性和其他免疫反应基因的过度表达可以作为靶点，以了解免疫基因如何调控植物生长的反应。此外，光合作用相关基因在矮化系中表达水平非常低。与植物生长相关的转录组表达和分类基因可能是研究植物生长的研究人员的有用资源。
