经典免疫蛋白质组学：用于抗原鉴定的血清蛋白质组分析（SERPA）

Classical Immunoproteomics: Serological Proteome Analysis (SERPA) for Antigen Identification.

作者信息

Fulton Kelly M, Ananchenko Anna, Wolfraim Lawrence, Martin Shannon, Twine Susan M

机构信息

Human Health Therapeutics Research Centre, National Research Council of Canada, Ottawa, ON, Canada.

Dynport Vaccine Company LLC, Frederick, MD, USA.

出版信息

Methods Mol Biol. 2019;2024:59-78. doi: 10.1007/978-1-4939-9597-4_3.

DOI:10.1007/978-1-4939-9597-4_3

PMID:31364042

Abstract

The study of the humoral immune response to infectious and chronic diseases is important for understanding the disease progression, identification of protective antigens, vaccine development, and discovery of biomarkers for early diagnosis. Proteomic approaches, including serological proteome analysis (SERPA), have been used to identify the repertoire of immunoreactive proteins in various diseases. In this chapter, we provide an outline of the SERPA approach, using the analysis of sera from mice vaccinated with a live attenuated tularemia vaccine as an example.

摘要

研究针对感染性疾病和慢性疾病的体液免疫反应，对于理解疾病进展、鉴定保护性抗原、疫苗研发以及发现早期诊断生物标志物都很重要。蛋白质组学方法，包括血清蛋白质组分析（SERPA），已被用于鉴定各种疾病中免疫反应性蛋白质的全部组成。在本章中，我们以对接种了减毒活兔热病疫苗的小鼠血清分析为例，概述SERPA方法。