使用荧光素酶免疫沉淀系统（LIPS）检测人诺如病毒特异性抗体。

Detection of Human Norovirus-Specific Antibodies Using the Luciferase Immunoprecipitation System (LIPS) Assay.

作者信息

Tin Christine M, Sosnovtsev Stanislav V

机构信息

Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

出版信息

Methods Mol Biol. 2019;2024:137-152. doi: 10.1007/978-1-4939-9597-4_8.

DOI:10.1007/978-1-4939-9597-4_8

PMID:31364047

Abstract

The luciferase immunoprecipitation system (LIPS) assay is a liquid-phase immunoassay that quantitates antigen-specific serum antibodies by measuring luminescence emitted by the reporter enzyme Renilla luciferase (Ruc) fused to an antigen of interest. The LIPS assay can be utilized as a high-throughput and sensitive serological method for profiling serum antibodies recognizing diverse antigens. This chapter provides a detailed protocol for detecting human norovirus-specific serum antibodies with the LIPS assay.

摘要

荧光素酶免疫沉淀系统（LIPS）检测是一种液相免疫测定法，通过测量与感兴趣抗原融合的报告酶海肾荧光素酶（Ruc）发出的发光来定量抗原特异性血清抗体。LIPS检测可作为一种高通量、灵敏的血清学方法，用于分析识别多种抗原的血清抗体。本章提供了使用LIPS检测法检测人诺如病毒特异性血清抗体的详细方案。