Department of Urinary Surgery, The Second Hospital of Yulin, Yulin, China.
Eur Rev Med Pharmacol Sci. 2019 Jul;23(14):6070-6078. doi: 10.26355/eurrev_201907_18420.
LINC00460 has been confirmed to contribute to cancer development. However, the role and function of LINC00460 in prostate cancer is not identified. The purpose of this study was to evaluate the expression and effect of LINC00460 on prostate cancer cell malignant behaviors.
The expression of LINC00460 in cancer tissues and cell lines were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) assay. The LINC00460 expression was downregulated by siRNA. The cell counting kit-8 (CCK-8) assay was used to detect cell proliferation. The cell migration and invasion were detected by migration and Matrigel invasion assays. The Western blot assay was used to detect the altered expression levels of Ki67, Cyclin D1, PI3K, p-AKT, T-AKT, Bcl2, and Bax.
LINC00460 was increased in human prostate cancer tissues and cell lines. LINC00460 high expression was related to Tumor Size (T1-T2/T3-T4; p=0.004), and high Gleason Score (≤8/>8, p=0.000). Downregulation of LINC00460 by siRNA could inhibit cancer cell proliferation and decreased Ki67 and Cyclin D1 expression. Meanwhile, downregulation of LINC00460 promoted apoptosis of cell lines and was related to PI3K/AKT pathway.
LINC00460 could regulate cell proliferation and cell apoptosis, which might be a novel marker in prostate cancer.
已经证实 LINC00460 有助于癌症的发展。然而，LINC00460 在前列腺癌中的作用和功能尚未确定。本研究旨在评估 LINC00460 对前列腺癌细胞恶性行为的表达和影响。
通过实时定量聚合酶链反应（qRT-PCR）检测 LINC00460 在癌组织和细胞系中的表达。通过 siRNA 下调 LINC00460 的表达。用细胞计数试剂盒-8（CCK-8）检测细胞增殖。用迁移和 Matrigel 侵袭试验检测细胞迁移和侵袭。Western blot 检测 Ki67、Cyclin D1、PI3K、p-AKT、T-AKT、Bcl2 和 Bax 的表达水平变化。
LINC00460 在人前列腺癌组织和细胞系中表达增加。LINC00460 的高表达与肿瘤大小（T1-T2/T3-T4；p=0.004）和高 Gleason 评分（≤8/>8，p=0.000）有关。siRNA 下调 LINC00460 可抑制癌细胞增殖，并降低 Ki67 和 Cyclin D1 的表达。同时，下调 LINC00460 促进细胞系凋亡，并与 PI3K/AKT 通路有关。
LINC00460 可调节细胞增殖和细胞凋亡，可能是前列腺癌的一种新标志物。
