School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
J Food Biochem. 2019 Nov;43(11):e12989. doi: 10.1111/jfbc.12989. Epub 2019 Jul 30.
The objective of this study was to understand the changes of nutrition constituents in extracts of four varieties of barley fermented with Lactobacillus plantarum dy-1 (LFBEs) and to uncover the potential apoptosis-related mechanism induced by LFBE to inhibit the proliferation of HT-29 cells. The contents of total polysaccharide, polyphenol, and protein in the four LFBEs significantly changed as the fermentation time went by and exerted different inhibitory effects on the proliferation of HT-29 cells. Results indicated that LFBE (YangSi No.3) inhibited proliferation of HT-29 cells in a time- and dose-dependent manners. The scanning electron micrograph illustrated that LFBE caused representative apoptotic trait and flow cytometric analysis suggested that LFBE brought about apoptosis by ceasing cell cycle at S phase. Western-blotting results indicated that LFBE promoted apoptosis was relevant to the regulation of apoptosis-related proteins, such as B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), and the release of Cytochrome-C from mitochondria. PRACTICAL APPLICATIONS: Abundant studies have reported that extracts of fermented barley held the activities of anti-obesity, antitumor, and so on. However, little information about the comparison in the chemical profile and antiproliferation property among different barley varieties (namely, YangSi barley No.1, YangSi barley No.3, DaZhong 88-91, XiYin No.2) was observed. Results indicated that LFBE (YangSi No.3 barley) exhibited the best inhibitory property by inducing the apoptosis of HT-29 cells. These findings may be beneficial to select a higher nutritional value barley and optimize the fermentation conditions to maximize the bioactive concentration expected in foods for the human.
本研究旨在了解四种不同品种的大麦经植物乳杆菌 dy-1（LFBE）发酵后提取物中营养成分的变化，并揭示 LFBE 抑制 HT-29 细胞增殖的潜在凋亡相关机制。四种 LFBE 中的总多糖、多酚和蛋白质含量随发酵时间的变化而显著变化，并对 HT-29 细胞的增殖产生不同的抑制作用。结果表明，LFBE（阳丝 3 号）呈时间和剂量依赖性抑制 HT-29 细胞的增殖。扫描电子显微镜图显示 LFBE 导致了典型的凋亡特征，流式细胞术分析表明 LFBE 通过使细胞周期停滞在 S 期而引发细胞凋亡。Western-blotting 结果表明，LFBE 促进凋亡与凋亡相关蛋白的调节有关，如 B 细胞淋巴瘤-2（Bcl-2）、Bcl-2 相关 X 蛋白（Bax）和细胞色素 C 从线粒体释放。实际应用：大量研究报道，发酵大麦提取物具有抗肥胖、抗肿瘤等活性。然而，关于不同大麦品种（即阳丝 1 号、阳丝 3 号、大种 88-91、西银 2 号）的化学成分谱和增殖抑制特性的比较信息很少。结果表明，LFBE（阳丝 3 号大麦）通过诱导 HT-29 细胞凋亡表现出最佳的抑制作用。这些发现可能有助于选择具有更高营养价值的大麦，并优化发酵条件，使食品中预期的生物活性浓度最大化，以满足人类的需求。
