School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China.
Biomed Environ Sci. 2019 Oct;32(10):755-768. doi: 10.3967/bes2019.095.
To further explore associated effects of Lactobacillus plantarum dy-1 (LFBE) on obesity and lipid metabolism at the gene expression level, the expression of microRNAs (miRNAs) was investigated in the liver of high-fat diet (HFD) induced obese rats.
Three groups of animal models were established. Changes in miRNA expression in the liver of each group were analyzed by microarray and RT-qPCR, complemented by bioinformatics. Palmitateinduced hepatocellular carcinoma (HepG2) cells were used as a model to validate the test.
LFBE treatment groups and HFD groups were observed to be distinctly different with respect to rates of increase in body weight and body fat percentage and triglyceride (TG) and total cholesterol (TC) levels in serum and liver. In addition, the LFBE group showed upregulation of ten miRNAs and downregulation of five miRNAs in the liver. Downregulation of miR-34a and miR-212 was observed in the livers of the LFBE group. Gene ontology and kyoto encyelopedia of geues and genomes (KEGG) pathway analysis showed that possible target genes of the deregulated miRNAs were significantly enriched in the adrenergic and HIF-1 signaling pathways.
These results demonstrate that LFBE might regulate the expression of miRNAs in order to inhibit obesity and fatty liver.
进一步探讨植物乳杆菌 dy-1（LFBE）在基因表达水平上对肥胖和脂代谢的相关影响，本研究检测了高脂肪饮食（HFD）诱导肥胖大鼠肝脏中 microRNAs（miRNAs）的表达情况。
建立了三组动物模型。通过微阵列和 RT-qPCR 分析了各组肝脏中 miRNA 表达的变化，并用生物信息学进行了补充。采用棕榈酸诱导的肝癌（HepG2）细胞作为模型进行验证实验。
与 HFD 组相比，LFBE 治疗组大鼠的体重和体脂百分比以及血清和肝脏中的甘油三酯（TG）和总胆固醇（TC）水平的增加率明显不同。此外，LFBE 组肝脏中存在 10 个 miRNA 的上调和 5 个 miRNA 的下调。LFBE 组肝脏中 miR-34a 和 miR-212 的下调。基因本体论和京都基因与基因组百科全书（KEGG）通路分析表明，失调 miRNA 的可能靶基因在肾上腺素能和 HIF-1 信号通路中显著富集。
这些结果表明 LFBE 可能通过调节 miRNA 的表达来抑制肥胖和脂肪肝。
