School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China.
Biomed Environ Sci. 2018 Sep;31(9):667-676. doi: 10.3967/bes2018.091.
To investigate the effect of fermented barley extracts with Lactobacillus plantarum dy-1 (LFBE) for modulating glucose consumption in HepG2 cells via miR-212 regulation.
Hepatocellular carcinoma (HepG2) cells were treated with palmitate. After 12 h, palmitate-induced HepG2 cells were treated with LFBE and its main components. Changes in glucose consumption, proinflammatory cytokine secretion, and miRNA-212 expression in HepG2 cells was observed.
Treatment with LFBE rich in vanillic acid (VA) increased glucose consumption and reduced proinflammatory cytokine secretion in HepG2 cells. LFBE and VA normalized the upregulation of miR-212, which led to the upregulation of dual-specificity phosphatase-9 (DUSP9), a direct target of miR-212, at both protein and mRNA levels. Downregulation of miR-212 markedly increased glucose consumption and reduced proinflammatory cytokine secretion by enhancing DUSP9 expression.
The results showed the benefit of LFBE and miR-212 downregulation in modulating glucose consumption and reducing proinflammatory cytokine secretion by targeting DUSP9. VA in LFBE was a strong regulator of palmitate-induced abnormal glucose consumption in HepG2 cells and can be a primary mediator.
通过 miR-212 调控，研究含植物乳杆菌 dy-1（LFBE）的发酵大麦提取物对 HepG2 细胞葡萄糖消耗的调节作用。
用棕榈酸处理肝癌（HepG2）细胞。12 h 后，用 LFBE 及其主要成分处理棕榈酸诱导的 HepG2 细胞。观察 HepG2 细胞葡萄糖消耗、促炎细胞因子分泌和 miR-212 表达的变化。
富含香草酸（VA）的 LFBE 处理可增加 HepG2 细胞的葡萄糖消耗，减少促炎细胞因子的分泌。LFBE 和 VA 使 miR-212 的上调正常化，导致 miR-212 的直接靶标双特异性磷酸酶-9（DUSP9）在蛋白和 mRNA 水平上调。下调 miR-212 通过增强 DUSP9 表达显著增加葡萄糖消耗并减少促炎细胞因子的分泌。
结果表明 LFBE 和 miR-212 下调通过靶向 DUSP9 调节葡萄糖消耗和减少促炎细胞因子分泌具有益处。LFBE 中的 VA 是 HepG2 细胞中棕榈酸诱导异常葡萄糖消耗的强调节剂，可作为主要介质。
