Huang Yan, Tang Xiaoying, Cehreli Zafer C, Dai Xiaoyun, Xu Jiangjingjun, Zhu Hongshui
Affiliated Stomatological Hospital of Nanchang University, The Key Laboratory of Oral Biomedicine, Department of Pediatric Dentistry, Jiangxi, PR China.
Department of Pediatric Dentistry, Louisiana State University Health Sciences Center School of Dentistry, New Orleans, LA, USA.
J Int Med Res. 2019 Oct;47(10):5094-5105. doi: 10.1177/0300060519862094. Epub 2019 Jul 31.
To investigate the potential for pulpal regeneration via autologous transplantation of deciduous tooth pulp into immature necrotic permanent teeth using an experimental dog model.
Experimental apical periodontitis was induced in 60 teeth of six Beagle dogs. Following canal disinfection and pulpotomy, autologous deciduous pulp tissue was transplanted into the root canals (n = 30); as controls, contralateral teeth were treated in accordance with the recommendations of the American Association of Endodontists. Radiographic examinations were performed immediately before transplant, as well as 3 and 6 months after transplant. At the 6-month examination, root samples were collected and histological and immunohistochemical analyses were used to examine tissue regeneration.
Radiographic analysis showed no significant differences in most histopathological parameters examined; however, apical diameter reduction was greater in the experimental group. Histological and immunohistochemical analyses showed that the canal walls of the experimental group had newly formed dentin-like tissue with dentinal tubules, while the control group had cementum-like deposits along the canal wall and apical foramina.
Autologous transplantation may be useful for regeneration of dental pulp in necrotic young permanent teeth.
使用实验犬模型研究通过将乳牙牙髓自体移植到未成熟坏死恒牙中实现牙髓再生的可能性。
在6只比格犬的60颗牙齿上诱导实验性根尖周炎。根管消毒和牙髓切断术后，将自体乳牙牙髓组织移植到根管中（n = 30）；作为对照，对侧牙齿按照美国牙髓病学家协会的建议进行治疗。在移植前以及移植后3个月和6个月进行影像学检查。在6个月检查时，收集牙根样本并进行组织学和免疫组织化学分析以检查组织再生情况。
影像学分析显示，在所检查的大多数组织病理学参数上无显著差异；然而，实验组的根尖直径减小更大。组织学和免疫组织化学分析表明，实验组的根管壁有新形成的类似牙本质的组织且有牙本质小管，而对照组沿根管壁和根尖孔有类牙骨质沉积。
自体移植可能有助于坏死年轻恒牙牙髓的再生。
