Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
J Med Microbiol. 2019 Sep;68(9):1359-1366. doi: 10.1099/jmm.0.001045. Epub 2019 Jul 31.
. Biochemical and molecular methods were used to identify 100 lactobacilli isolated from rectal swabs. Among these, ssp. LP5 and LP9 showed significant antibacterial activity against and . Accordingly, characterization of their bacteriocins, BacLP5 and BacLP9, was conducted to obtain information on their kinetic production, sensitivity to chemico-physical parameters and molecular weight. To investigate the possible use of the two strains as probiotics, their gastrointestinal resistance, cellular adhesiveness and sensitivity to antibiotics were also studied.. The obtained data show that BacLP5 and BacLP9 most likely belong to class II bacteriocins and both have a molecular weight of approximately 3 kDa. The production of BacLP5 and BacLP9 started after 4 h (40 and 80 AU ml), respectively. Both of the strains survived gastric and intestinal juices well and showed adhesive capability on HEp-2 cells.. Due to their peculiar antimicrobial characteristics, ssp. LP5 and LP9 are suitable for use in the treatment of vaginal disorders, through both oral and transvaginal administration.
采用生化和分子方法鉴定了 100 株从直肠拭子中分离出的乳杆菌。其中，ssp. LP5 和 LP9 对 和 表现出显著的抗菌活性。因此，对其细菌素 BacLP5 和 BacLP9 进行了表征，以获取关于其动力学产生、对化学-物理参数和分子量的敏感性的信息。为了研究这两种菌株作为益生菌的可能用途，还研究了它们的胃肠道抗性、细胞粘附性和对抗生素的敏感性。获得的数据表明，BacLP5 和 BacLP9 很可能属于 II 类细菌素，分子量均约为 3kDa。BacLP5 和 BacLP9 的产生分别在 4 小时（40 和 80 AU ml）后开始。两种菌株都能很好地耐受胃液和肠液，并且对 HEp-2 细胞具有粘附能力。由于其独特的抗菌特性，ssp. LP5 和 LP9 适合通过口服和阴道给药来治疗阴道疾病。
