State Key Laboratory of Food Science and Technology , Nanchang University , 235 Nanjing East Road , Nanchang , Jiangxi 330047 , People's Republic of China.
Jiangxi Yeli Medical Device Company, Limited , 2799 Tianxiang Avenue , Nanchang , Jiangxi 330008 , People's Republic of China.
J Agric Food Chem. 2019 Aug 21;67(33):9390-9398. doi: 10.1021/acs.jafc.9b03414. Epub 2019 Aug 8.
Various pathogens may coexist in one sample; however, detection methods that rely on traditional selective culture media or immune agents designed specifically for a certain target are unsuitable for multiple targets. It is important to develop a simultaneous and sensitive detection method for multiple pathogens. Here, a multicolor and ultrasensitive enzyme-linked immunosorbent assay (ELISA) platform based on the fluorescence hybridization chain reaction (HCR) was developed. In the assay, multicolor fluorescence concatemers formed as signal amplifiers and signal reporters in the presence of target pathogens. When HCR occurred, O157:H7, serotype Choleraesuis, and were detected simultaneously with three different fluorescences. Additionally, the limits of detection for O157:H7, Choleraesuis, and were 3.4 × 10, 6.4 × 10, and 7.0 × 10 CFU/mL, respectively. The assay achieved ultrasensitive, specific, and simultaneous detection of three pathogens and can be applied to the detection of pathogens in milk samples. Therefore, this multicolor and ultrasensitive ELISA platform has great potential in the application of simultaneous detection of pathogens.
多种病原体可能共存于一个样本中；然而，依赖于传统的选择性培养基或专门针对特定靶标的免疫剂的检测方法不适合多个靶标。因此，开发一种用于多种病原体的同时且敏感的检测方法非常重要。在这里，开发了一种基于荧光杂交链式反应 (HCR) 的多色和超灵敏酶联免疫吸附测定 (ELISA) 平台。在该测定中，多色荧光串联物在存在目标病原体的情况下形成信号放大器和信号报告物。当 HCR 发生时，O157:H7、血清型霍乱弧菌和 可以同时用三种不同的荧光进行检测。此外，O157:H7、霍乱弧菌和 的检测限分别为 3.4×10、6.4×10 和 7.0×10 CFU/mL。该测定实现了对三种病原体的超灵敏、特异性和同时检测，可应用于牛奶样品中病原体的检测。因此，这种多色和超灵敏的 ELISA 平台在病原体的同时检测应用中具有很大的潜力。
