Maurer D H, Callaway C, Sorkin S, Pollack M S
Department of Microbiology and Immunology, Baylor College of Medicine, Methodist Hospital, Houston, Texas.
Tissue Antigens. 1988 Apr;31(4):174-82. doi: 10.1111/j.1399-0039.1988.tb02079.x.
Cultured amniotic fluid cells, which are used for HLA typing studies for the prenatal diagnosis of HLA linked diseases and for prenatal determination of paternity, usually contain mixtures of fibroblastic and epithelioid cells. Cells of both types lack constitutive expression of HLA class II antigens, but these can be induced by pretreatment with gamma interferon. Both serological and functional studies indicate that detectable levels of DR and DP but not DQ antigens can be thereby induced. DR and DP cannot, however, be induced on chorionic villus cells.
培养的羊水细胞通常包含成纤维细胞和上皮样细胞的混合物，这些细胞用于HLA分型研究，以进行HLA相关疾病的产前诊断和产前亲子鉴定。这两种类型的细胞都缺乏HLA II类抗原的组成性表达，但可以通过γ干扰素预处理来诱导表达。血清学和功能研究均表明，由此可以诱导出可检测水平的DR和DP抗原，但不能诱导出DQ抗原。然而，DR和DP抗原不能在绒毛膜绒毛细胞上诱导表达。
