大黄素对哒嗪磷暴露下草鱼（Ctenopharyngodon idellus）ABC 转运体基因表达的影响。
Effects of emodin on ABC transporter gene expression in grass carp (Ctenopharyngodon idellus) exposed to diazinon.
机构信息
Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture and Rural Affairs; East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
出版信息
PLoS One. 2019 Jul 31;14(7):e0219866. doi: 10.1371/journal.pone.0219866. eCollection 2019.
This study aimed to investigate the function of ATP-binding cassette (ABC) transporter genes in grass carp treated with emodin combined with diazinon (DZN) exposure. The transcription levels of five ABC transporter genes in different tissues of grass carp and at different time points were measured by real-time quantitative PCR (qRT-PCR). The analysis of different tissues showed higher ABCB1 expression in the skin (26-fold) and gill (2-fold) than in the liver. In addition, ABCB11 expression was higher in the skin (109-fold) and gill (57-fold) than in the liver, ABCC1 was more highly expressed in the gill (50-fold) than in the liver, and ABCG2 was expressed at higher levels in the skin (659-fold, p < 0.01), gill (628-fold, p < 0.01) and liver (659-fold, p < 0.01) than in brain tissue. The analysis of different time points revealed that the ABCB1, ABCB11, ABCC1, ABCC2 and ABCG2 genes were highly expressed at 24 h in the liver in the experimental group. However, analysis of the intestinal tissue of the experimental group showed that the expression of ABCB1 and ABCB11 peaked at 6 h, the expression of ABCC1 and ABCC2 peaked at 5 d, and the expression of ABCG2 peaked at 3 d. Furthermore, the emodin concentrations in the liver and intestine reached their peak levels (50.18 and 117.24 μg·ml-1, respectively) after 48 and 1 h of treatment with emodin combined with DZN, respectively. The peak DZN concentrations in the liver (1.42 ng·ml-1) and intestine (0.2 ng·ml-1) were detected 3 and 6 h after emodin treatment combined with DZN, respectively. In conclusion, this study shows that the transcript levels of ABC transporters respond to the presence of emodin, which indicates their potential involvement in and contribution with the metabolic process in grass carp.
本研究旨在探讨 ATP 结合盒（ABC）转运体基因在大黄素联合敌敌畏处理草鱼中的功能。通过实时定量 PCR（qRT-PCR）测量了草鱼不同组织和不同时间点的 5 种 ABC 转运体基因的转录水平。不同组织的分析表明，皮肤（26 倍）和鳃（2 倍）中 ABCB1 的表达高于肝脏。此外，皮肤（109 倍）和鳃（57 倍）中 ABCB11 的表达高于肝脏，ABCC1 在鳃（50 倍）中的表达高于肝脏，ABCG2 在皮肤（659 倍，p <0.01）、鳃（628 倍，p <0.01）和肝脏（659 倍，p <0.01）中的表达水平高于脑组织。不同时间点的分析表明，实验组肝脏中 ABCB1、ABCB11、ABCC1、ABCC2 和 ABCG2 基因在 24 h 时高度表达。然而，实验组肠组织分析表明，ABCB1 和 ABCB11 的表达在 6 h 时达到峰值，ABCC1 和 ABCC2 的表达在 5 d 时达到峰值，ABCG2 的表达在 3 d 时达到峰值。此外，大黄素联合敌敌畏处理后 48 h 和 1 h，肝脏和肠道中大黄素的浓度分别达到峰值（50.18 和 117.24 μg·ml-1）。大黄素联合敌敌畏处理后 3 h 和 6 h，肝脏（1.42 ng·ml-1）和肠道（0.2 ng·ml-1）中敌敌畏的浓度分别达到峰值。综上所述，本研究表明，ABC 转运体的转录水平对大黄素的存在有反应，这表明它们可能参与并有助于草鱼的代谢过程。
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