Halaji Mehrdad, Hashempour Tayebeh, Moayedi Javad, Pouladfar Gholam Reza, Khansarinejad Behzad, Khashei Reza, Moattari Afagh, Musavi Zahra, Ghassabi Farzaneh, Pirbonyeh Neda
Clinical Microbiology Research Center, Nemazee Teaching Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Microbiology & Immunology, Arak University of Medical Sciences, Arak, Iran.
Rev Soc Bras Med Trop. 2019 Jul 29;52:e20180249. doi: 10.1590/0037-8682-0249-2018.
Prevalence of influenza A virus (Flu-A), respiratory syncytial virus (RSV), and human metapneumovirus (hMPV) was assessed in children with acute respiratory infections (ARIs).
Nasopharyngeal aspirates and throat swabs were subjected to real-time polymerase chain reaction (PCR) to detect RSV and Flu-A and to conventional PCR to detect hMPV.
Of the 156 children assessed, 93 (59.6%) carried at least one virus, with 35.9% positive for RSV, 14.1% for hMPV, and 9.6% for Flu-A. The prevalence of co-infections was 2.6%.
The high detection rate may reflect increased sensitivity of real-time PCR compared to traditional PCR and viral culture.
对患有急性呼吸道感染（ARI）的儿童进行了甲型流感病毒（Flu-A）、呼吸道合胞病毒（RSV）和人偏肺病毒（hMPV）的流行率评估。
对鼻咽抽吸物和咽拭子进行实时聚合酶链反应（PCR）以检测RSV和Flu-A，并用常规PCR检测hMPV。
在评估的156名儿童中，93名（59.6%）携带至少一种病毒，其中RSV阳性率为35.9%，hMPV为14.1%，Flu-A为9.6%。合并感染的流行率为2.6%。
高检出率可能反映了与传统PCR和病毒培养相比，实时PCR的灵敏度有所提高。
