Department of Plant Science, McGill University, Macdonald Campus, 21111 Lakeshore, Ste-Anne-de-Bellevue, Québec H9X 3V9, Canada; Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal, Québec H1X 2B2, Canada.
Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, Montréal, Québec H1X 2B2, Canada.
Cell Rep. 2019 Jul 30;28(5):1237-1250.e6. doi: 10.1016/j.celrep.2019.07.006.
Pavement cells form wavy interlocking patterns in the leaf epidermis of many plants. We use computational mechanics to simulate the morphogenetic process based on microtubule organization and cell wall chemistry. Based on the in silico simulations and experimental evidence, we suggest that a multistep process underlies the morphogenesis of pavement cells. The in silico model predicts alternatingly located, feedback-augmented mechanical heterogeneity of the periclinal and anticlinal walls. It suggests that the emergence of waves is created by a stiffening of the emerging indented sides, an effect that matches cellulose and de-esterified pectin patterns in the cell wall. Further, conceptual evidence for mechanical buckling of the cell walls is provided, a mechanism that has the potential to initiate wavy patterns de novo and may precede chemical and geometrical symmetry breaking.
pavement cells 在许多植物的叶表皮中形成波浪状交错的图案。我们使用计算力学基于微管组织和细胞壁化学来模拟形态发生过程。基于计算机模拟和实验证据，我们提出了 pavement cells 形态发生的多步过程。计算机模型预测了沿垂周壁和切向壁交替定位、反馈增强的机械异质性。它表明，波的出现是由新兴的凹入侧的硬化产生的，这种效果与细胞壁中的纤维素和去酯化果胶模式相匹配。此外，还提供了细胞壁机械屈曲的概念证据，这种机制有可能从头开始引发波浪图案，并可能先于化学和几何对称破缺。
