*Department of Medicine Division of Chest Medicine, Cheng Ching Hospital, No. 966, Sec. 4, Taiwan Road, Taichung, Taiwan.
†School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, No. 91, Hsueh-Shih Road, Taichung, Taiwan.
Am J Chin Med. 2019;47(5):1099-1112. doi: 10.1142/S0192415X19500563. Epub 2019 Jul 31.
Asthma is the most prevalent chronic respiratory disease worldwide. Garlic extracts have long been used as a food source and in traditional medicine. Crude extracts of garlic are used as an anti-inflammatory agent and have been reported to exhibit antiasthmatic properties. However, molecular mechanisms of garlic extracts in the context of antiasthmatic airway inflammation are still unclear. In this study, the antiasthmatic effect of garlic extracts on Th1, Th2, and Th3 cytokine profiles and immunoregulatory mechanism were explored using an animal model of allergic asthma. Garlic extracts significantly reduced total inflammatory cell counts and eosinophil infiltration and decreased the production of IgE in serum and Th1/Th2/Th3 cytokine in bronchoalveolar fluid. Enzyme-linked immunosorbent assay analysis demonstrated that garlic extracts downregulated the levels of cytokines and chemokines, namely Th2-related IL-4, IL-5, and IL-13; but they simultaneously upregulated Th1-related IFN-, IL-12, and Th3-related IL-10 and TGF- expression in BALF. The mechanism may be ascribed to the modulation of Th1-, Th2-, and Th3-related cytokine imbalance.
哮喘是全球最常见的慢性呼吸道疾病。大蒜提取物长期以来一直被用作食物来源和传统医学中的药物。大蒜的粗提取物被用作抗炎剂，据报道具有抗哮喘特性。然而，大蒜提取物在抗哮喘气道炎症方面的分子机制尚不清楚。在这项研究中，我们使用过敏性哮喘动物模型探索了大蒜提取物对 Th1、Th2 和 Th3 细胞因子谱及免疫调节机制的抗哮喘作用。大蒜提取物可显著降低总炎性细胞计数和嗜酸性粒细胞浸润，并降低血清 IgE 和支气管肺泡灌洗液中 Th1/Th2/Th3 细胞因子的产生。酶联免疫吸附试验分析表明，大蒜提取物可下调细胞因子和趋化因子的水平，即 Th2 相关的 IL-4、IL-5 和 IL-13；同时，还可上调 BALF 中 Th1 相关的 IFN-γ、IL-12 和 Th3 相关的 IL-10 和 TGF-β的表达。其机制可能与 Th1、Th2 和 Th3 相关细胞因子失衡的调节有关。
