Costa Camila Medeiros, Santos Matheus Augusto Teixeira Dos, Pernambuco Andrei Pereira
Department of Physiotherapy, Centro Universitário de Formiga UNIFOR-MG , Minas Gerais , Brazil.
Department of Physiotherapy, Universidade de Itaúna (UIT) , Minas Gerais , Brazil.
J Immunoassay Immunochem. 2019;40(5):540-554. doi: 10.1080/15321819.2019.1649695. Epub 2019 Jul 31.
Rheumatoid arthritis (RA) is an autoimmune and progressive disease. Evidence indicates that inflammatory mediators may contribute to the genesis and/or evolution of this clinical condition. Thus, the objective was to evaluate and compare the plasma levels of Interleukin-17 (IL-17), Tumor Necrosis Factor-Alpha (TNF-α) and Complement 3 (C3) in women with RA and healthy controls (HC), as well as to evaluate the association them with the disease activity. 25 women with RA and 15 HC were recruited. Plasma levels of biomarkers were measured by ELISA. All statistical analyzes were performed with a significance level set at α = 0.05. In the women with RA, the median age was 55 and, in the HC, was 50 years. The median value of DAS-28 was 3.79. The plasma levels of IL-17 ( = .03), TNF-α (p ≤ 0.01) and C3 (p ≤ 0.01) were higher in women with RA. The ROC curve showed that TNF- α has a higher discriminating ability than IL-17 and C3. DAS-28 score correlated significantly with C3 levels in women with RA (r = 0.91; < .01). These findings reaffirm the participation of the immune system in pathophysiology of RA, suggest that TNF-α levels may be a good biomarker and that elevated C3 levels contribute to the worsening of the disease.
类风湿性关节炎（RA）是一种自身免疫性进展性疾病。有证据表明，炎症介质可能促成这种临床病症的发生和/或演变。因此，本研究的目的是评估和比较类风湿性关节炎女性患者与健康对照者（HC）血浆中白细胞介素-17（IL-17）、肿瘤坏死因子-α（TNF-α）和补体3（C3）的水平，并评估它们与疾病活动度的相关性。招募了25名类风湿性关节炎女性患者和15名健康对照者。采用酶联免疫吸附测定法（ELISA）测量生物标志物的血浆水平。所有统计分析的显著性水平设定为α = 0.05。类风湿性关节炎女性患者的中位年龄为55岁，健康对照者的中位年龄为50岁。DAS-28的中位值为3.79。类风湿性关节炎女性患者的IL-17（p = 0.03）、TNF-α（p≤0.01）和C3（p≤0.01）血浆水平较高。ROC曲线显示，TNF-α的鉴别能力高于IL-17和C3。在类风湿性关节炎女性患者中，DAS-28评分与C3水平显著相关（r = 0.91；p < 0.01）。这些发现再次证实了免疫系统参与类风湿性关节炎的病理生理过程，提示TNF-α水平可能是一个良好的生物标志物，且C3水平升高会导致疾病恶化。
