Inagawa Hiroyuki, Nishizawa Takashi, Kochi Chie, Amano Satoko, Soma Gen-Ichiro
Control of Innate Immunity, Technology Research Association, Kagawa, Japan
Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan.
Anticancer Res. 2019 Aug;39(8):4511-4516. doi: 10.21873/anticanres.13627.
BACKGROUND/AIM: Gluconacetobacter hansenii (G. hansenii) is an acetic acid bacterium of vinegar production. Its anti-allergic effect on mice upon oral administration was examined.
The amount of LPS was measured by the Limulus reaction. Mice were sensitized by peritoneal and intranasal administration of cedar pollen and alum followed by oral administration of 30 or 150 mg/kg of heated G. hansenii cells. Pollen was administered intranasally to evaluate nasal symptoms, and at 8 weeks, IgE and IL-10 levels in blood were measured by ELISA.
The amount of LPS in dried bacterial cells was 10.4±3.3 mg/g. In the cedar pollinosis model of mice, a significant reduction was observed in nose scratching of both groups administered with the bacterial cells (30, 150 mg/kg).
G. hansenii contains LPS, and its oral administration showed an anti-allergic effect by a significant mitigation of the symptoms in a pollen allergy mouse model.
背景/目的：汉逊葡糖酸杆菌（G. hansenii）是一种用于生产醋的醋酸菌。研究了其口服给药对小鼠的抗过敏作用。
通过鲎试剂反应测定脂多糖（LPS）含量。通过腹腔和鼻内给予雪松花粉和明矾使小鼠致敏，随后口服30或150mg/kg加热的G. hansenii细胞。鼻内给予花粉以评估鼻部症状，并在8周时通过酶联免疫吸附测定法（ELISA）测量血液中的免疫球蛋白E（IgE）和白细胞介素-10（IL-10）水平。
干燥细菌细胞中的LPS含量为10.4±3.3mg/g。在小鼠雪松花粉症模型中，给予细菌细胞（30、150mg/kg）的两组小鼠的挠鼻次数均显著减少。
G. hansenii含有LPS，其口服给药通过显著减轻花粉过敏小鼠模型中的症状而显示出抗过敏作用。
