Maejima K, Tamura K, Taniguchi Y, Nagase S, Tanaka H
Japan Automobile Research Institute, Ibaraki, Japan.
J Toxicol Environ Health. 1997 Oct 24;52(3):231-48. doi: 10.1080/00984109708984062.
The adjuvant effects of various fine particles [Kanto loam dust, fly ash, carbon black, diesel exhaust particles (DEP), and aluminum hydroxide (alum)] on immunoglobulin E (IgE) antibody production in female BDF1 mice were examined. In experiment 1, animals both received 25 micrograms of each particle intranasally and were exposed to aerosolized Japanese cedar pollen allergens (JCPA) for 30 min/d at 1-wk intervals for the first 8 wk. This was followed by exposure for 30 min every 3 wk for the next 9 wk. As parameters of allergic rhinitis, measurements were made of JCPA-specific IgE and IgG antibody titers, the protein-adsorbing capacity of each type of particle, and nasal rubbing movements. The increases in anti-JCPA IgE and IgG antibody production in mice treated with aerosolized JCPA plus respective particles were significantly greater than that found with aerosolized JCPA alone. This was associated with no marked differences in the other allergic rhinitis parameters. In experiment 2, after the administration of particles as in experiment 1, about 160,000 grains of Japanese cedar pollen (JCP, native dry pollen) were dropped onto the tip of the nose of mice twice a week for 16 wk. Six weeks after the first immunization, the anti-JCPA IgE antibody titers of groups treated with the respective particles were greater than 1:20, whereas those of mice treated with JCP alone were 1:10. No significant differences in the anti-JCPA IgE and IgG antibody productions, nasal rubbing counts, or histopathological changes were observed after 18 wk. These results suggested the nature of the particles, their capacity to adsorb antigens, and/or their size may not be related to enhancement of IgG antibody production nor symptoms of allergic rhinitis. However, IgE antibody production seemed to occur earlier in mice treated with particles than in mice immunized with allergens alone.
研究了各种细颗粒物质（关东壤土灰尘、飞灰、炭黑、柴油尾气颗粒（DEP）和氢氧化铝（明矾））对雌性BDF1小鼠免疫球蛋白E（IgE）抗体产生的佐剂效应。在实验1中，给动物鼻腔内分别注射25微克每种颗粒，并在最初8周内每隔1周每天暴露于雾化的日本雪松花粉过敏原（JCPA）30分钟。接下来的9周内，每隔3周暴露30分钟。作为过敏性鼻炎的参数，检测了JCPA特异性IgE和IgG抗体滴度、每种颗粒的蛋白质吸附能力以及鼻摩擦动作。用雾化JCPA加相应颗粒处理的小鼠中，抗JCPA IgE和IgG抗体产生的增加显著大于单独使用雾化JCPA的小鼠。这与其他过敏性鼻炎参数无明显差异相关。在实验2中，按照实验1的方式给予颗粒后，每周两次将约160,000粒日本雪松花粉（JCP，天然干花粉）滴到小鼠鼻尖上，持续16周。首次免疫6周后，用相应颗粒处理的组的抗JCPA IgE抗体滴度大于1:20，而仅用JCP处理的小鼠的抗体滴度为1:10。18周后，未观察到抗JCPA IgE和IgG抗体产生、鼻摩擦次数或组织病理学变化的显著差异。这些结果表明，颗粒的性质、其吸附抗原的能力和/或其大小可能与IgG抗体产生的增强或过敏性鼻炎症状无关。然而，用颗粒处理的小鼠中IgE抗体产生似乎比仅用过敏原免疫的小鼠更早出现。
