在神经生长因子促进大鼠嗜铬细胞瘤PC12细胞神经突生长过程中聚（ADP - 核糖）合成酶水平的降低

Decrease in the level of poly(ADP-ribose) synthetase during nerve growth factor-promoted neurite outgrowth in rat pheochromocytoma PC12 cells.

作者信息

Taniguchi T, Morisawa K, Ogawa M, Yamamoto H, Fujimoto S

机构信息

Medical Research Laboratory, Kochi Medical School, Japan.

出版信息

Biochem Biophys Res Commun. 1988 Aug 15;154(3):1034-40. doi: 10.1016/0006-291x(88)90244-6.

DOI:10.1016/0006-291x(88)90244-6

PMID:3136766

Abstract

We have studied the changes in the levels of the enzyme molecule and mRNA for poly(ADP-ribose) synthetase during nerve growth factor-promoted neurite outgrowth in rat pheochromocytoma PC12 cells. When the PC12 cells were cultured in the presence of nerve growth factor, the content of enzyme molecules decreased along with neurite outgrowth to 50% of the original amounts in 2 days and the content of mRNA for the enzyme also decreased to approximately 50% in 2 days. These results suggest that the decrease of the enzyme molecule may be due to depression of expression of the gene for synthetase during the process. Taken together with previous observations, the decrease of the synthetase seems to be required for some cellular differentiation.

摘要

我们研究了在神经生长因子促进大鼠嗜铬细胞瘤PC12细胞神经突生长过程中，聚（ADP - 核糖）合成酶的酶分子和mRNA水平的变化。当PC12细胞在神经生长因子存在的情况下培养时，酶分子的含量随着神经突的生长在2天内降至原始含量的50%，并且该酶的mRNA含量在2天内也降至约50%。这些结果表明，酶分子的减少可能是由于在此过程中合成酶基因表达的抑制。结合先前的观察结果，合成酶的减少似乎是某些细胞分化所必需的。