Department of Surgery and Anatomy, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, SP, Brazil.
Barão de Maua University Center, Ribeirao Preto, SP, Brazil.
J Mol Neurosci. 2019 Dec;69(4):580-587. doi: 10.1007/s12031-019-01386-w. Epub 2019 Jul 31.
The aim of this study was to analyze the expression profiles of the microRNAs (miRNAs) miR-145, miR-181c, miR-199a and miR-1183 in the hippocampus and blood of patients with mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS) and to investigate whether these can be used as diagnosis and prognosis biomarkers for epilepsy. Hippocampus and blood samples were collected from 20 patients with MTLE-HS, ten of whom had a favorable surgical outcome (Engel I) and ten with an unfavorable surgical outcome (Engel III-IV). Hippocampus samples from autopsied individuals with no neurological or psychiatric medical history (necropsy samples) and blood samples from healthy individuals were used as controls. Real-time quantitative PCR (RQ-PCR) was used to analyze miRNA expression. The results showed that the expressions of these miRNAs differed quantitatively in the hippocampus and blood of patients with MTLE-HS in comparison to the respective control. This difference was most pronounced for miR-145, which was hypo-expressed in the hippocampus and hyper-expressed in the blood of MTLE-HS patients. MiRNAs miR-145, miR-181c, miR-199a and miR-1183 were hyper-expressed in the blood of patients with MTLE-HS. No statistical differences in the levels of these miRNAs in the blood or hippocampus were found between Engel I patients and Engel III-IV patients. These results suggest that the analyzed microRNAs are potential circulating biomarkers for epilepsy diagnosis.
本研究旨在分析海马和血液中微小 RNA（miRNA）miR-145、miR-181c、miR-199a 和 miR-1183 在伴有海马硬化的颞叶内侧癫痫（MTLE-HS）患者中的表达谱，并探讨这些 miRNA 是否可作为癫痫的诊断和预后生物标志物。从 20 例 MTLE-HS 患者中采集海马和血液样本，其中 10 例手术预后良好（Engel I 级），10 例手术预后不良（Engel III-IV 级）。使用无神经或精神病史的尸检个体的海马样本（尸检样本）和健康个体的血液样本作为对照。采用实时定量 PCR（RQ-PCR）分析 miRNA 表达。结果显示，与各自对照相比，MTLE-HS 患者的海马和血液中这些 miRNA 的表达存在定量差异。miR-145 的差异最为明显，在 MTLE-HS 患者的海马中呈低表达，在血液中呈高表达。miR-145、miR-181c、miR-199a 和 miR-1183 在 MTLE-HS 患者的血液中呈高表达。在 Engel I 级患者和 Engel III-IV 级患者的血液或海马中，这些 miRNA 的水平无统计学差异。这些结果表明，分析的 microRNAs 可能是癫痫诊断的潜在循环生物标志物。
