Imsil Cheese & Food Research Institute, Imsil-gun, Republic of Korea.
Department of Food Science and Technology, Chonbuk National University, Jeonju, Republic of Korea.
J Food Biochem. 2019 Nov;43(11):e12999. doi: 10.1111/jfbc.12999. Epub 2019 Jul 31.
Neurodegenerative diseases are major threats to human health. Here, through fluorescence, colorimetric, immunoblotting, spectroscopy, and laser scanning confocal microscopic techniques, we investigated the neuroprotective properties of chlorogenic acid-rich Solanum melongena extracts (SM extract) in rotenone-induced PC-12 cell death. The results showed that rotenone caused apoptosis to PC-12 cells by elevating Bax/Bcl-2 ratio and increasing caspase-3 activity. Rotenone also increased ROS in cells while suppressing SOD and catalase activities. This resulted in the depletion of ATP in cells by blocking mitochondria complex I activity. Pretreatment of the cells with SM extract at concentrations of 100, 250, and 500 μg/ml before incubation for 24 hr with rotenone significantly prevented apoptosis, decreased ROS, and increased ATP production in the cells. SM extract upregulated SOD and catalase activities in the cells. These results unveil evidence that SM extract content neuroprotective properties that can be exploited to prevent and treat neurodegenerative diseases. PRACTICAL APPLICATIONS: Solanum melongena eggplant is a popular ingredient in many traditional recipes and is well known in Asia for its medicinal benefits. Despite numerous scientific reports of the potential health benefits of this plant, reports on its effects in neurodegenerative diseases is still lacking. This pilot study demonstrates that S. melongena eggplant can protect against neurotoxicity in neurodegenerative diseases. The results of this research serves as a base for further research on eggplant that will result in its usage on a larger scale as functional food materials.
神经退行性疾病是人类健康的主要威胁。在这里，我们通过荧光、比色、免疫印迹、光谱和激光扫描共聚焦显微镜技术，研究了富含绿原酸的茄提取物（SM 提取物）在鱼藤酮诱导的 PC-12 细胞死亡中的神经保护特性。结果表明，鱼藤酮通过提高 Bax/Bcl-2 比值和增加 caspase-3 活性诱导 PC-12 细胞凋亡。鱼藤酮还增加了细胞内的 ROS，同时抑制了 SOD 和过氧化氢酶的活性。这导致通过阻断线粒体复合物 I 活性使细胞内的 ATP 耗竭。在用鱼藤酮孵育 24 小时之前，用浓度为 100、250 和 500μg/ml 的 SM 提取物预处理细胞，可显著防止细胞凋亡，降低 ROS，并增加细胞内的 ATP 产生。SM 提取物上调了细胞内的 SOD 和过氧化氢酶的活性。这些结果表明 SM 提取物具有神经保护作用，可以用来预防和治疗神经退行性疾病。实际应用：茄子是许多传统食谱中的常见成分，在亚洲因其药用功效而广为人知。尽管有大量关于这种植物潜在健康益处的科学报告，但关于其在神经退行性疾病中的作用的报告仍然缺乏。这项初步研究表明，茄子可以预防神经退行性疾病中的神经毒性。这项研究的结果为进一步研究茄子提供了基础，将导致其在更大规模上作为功能性食品材料使用。
