Department of Neurology, The Agnes Ginges Center for Human Neurogenetics, Hadassah - Hebrew University Medical Center, Jerusalem, Israel.
Department of Physical Therapy, Faculty of Health Sciences, Ariel University, Ariel, Israel.
Ann Clin Transl Neurol. 2019 Sep;6(9):1647-1658. doi: 10.1002/acn3.50859. Epub 2019 Aug 1.
Exercise training (ET) has beneficial effects on multiple sclerosis and its animal model experimental autoimmune encephalomyelitis (EAE). However, the intensity-dependent effects of ET on the systemic immune system in EAE remain undefined.
(1) To compare the systemic immune modulatory effects of moderate versus high-intensity ET protocols in protecting against development of EAE; (2) To investigate whether ET affects autoimmunity selectively, or causes general immunosuppression.
Healthy mice performed moderate or high-intensity treadmill running programs. Proteolipid protein (PLP)-induced transfer EAE was utilized to examine ET effects specifically on the systemic immune system. Lymph node (LN)-T cells from trained versus sedentary donor mice were transferred to naïve recipients and EAE severity was assessed, by clinical assessment and histopathological analysis. LN-T cells derived from donor trained versus sedentary PLP-immunized mice were analyzed in vitro for proliferation assays by flow cytometry analysis and cytokine and chemokine receptor gene expression using real-time PCR. T cell-dependent immune responses of trained versus sedentary mice to the nonautoantigen ovalbumin and susceptibility to Escherichia coli-induced acute peritonitis were examined.
High-intensity training in healthy donor mice induced significantly greater inhibition than moderate-intensity training on proliferation and generation of encephalitogenic T cells in response to PLP-immunization, and on EAE severity upon their transfer into recipient mice. High-intensity training also inhibited LN-T cell proliferation in response to ovalbumin immunization. E. coli bacterial counts and dissemination were not affected by training.
High-intensity training induces superior effects in preventing autoimmunity in EAE, but does not alter immune responses to E. coli infection.
运动训练（ET）对多发性硬化症及其动物模型实验性自身免疫性脑脊髓炎（EAE）有有益影响。然而，ET 对 EAE 中系统性免疫系统的强度依赖性影响尚不清楚。
（1）比较中等强度与高强度 ET 方案在预防 EAE 发展方面的系统性免疫调节作用；（2）研究 ET 是否选择性地影响自身免疫，还是导致一般性免疫抑制。
健康小鼠进行中等或高强度跑步机跑步计划。利用蛋白脂蛋白（PLP）诱导的转移 EAE 来专门检查 ET 对系统性免疫系统的影响。来自训练有素和久坐的供体小鼠的淋巴结（LN）-T 细胞被转移到未致敏的受体中，并通过临床评估和组织病理学分析评估 EAE 的严重程度。通过流式细胞术分析和实时 PCR 分析对来自供体训练有素和久坐 PLP 免疫小鼠的 LN-T 细胞进行体外增殖测定，以分析细胞因子和趋化因子受体基因表达。检查训练有素和久坐小鼠对非自身抗原卵清蛋白的 T 细胞依赖性免疫反应以及对大肠杆菌诱导的急性腹膜炎的易感性。
健康供体小鼠的高强度训练比中等强度训练更显著地抑制了对 PLP 免疫的增殖和致脑炎 T 细胞的产生，并且在将其转移到受体小鼠中时抑制了 EAE 的严重程度。高强度训练还抑制了对卵清蛋白免疫的 LN-T 细胞增殖。大肠杆菌细菌计数和传播不受训练影响。
高强度训练可更好地预防 EAE 中的自身免疫，但不会改变对大肠杆菌感染的免疫反应。
