Zhou Zi-Xian, Ma Hui-Fang, Yang Yi-Chen, Chen Jun-Tao, Feng Yuan-Hui
School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 102488, China.
Zhen Ci Yan Jiu. 2022 Jul 25;47(7):611-6. doi: 10.13702/j.1000-0607.20220031.
OBJECTIVE: To observe the effect of electroacupuncture (EA) of "Tianshu"(ST25) and "Shangjuxu"(ST37)on gastrointestinal motility, psychological abnormality and expression of RhoA and ROCK protein in rats with diarrhea predominant irritable bowel syndrome (IBS-D), so as to explore its underlying mechanism in improving IBS-D. METHODS: Thirty-six male rats were randomly and equally divided into control, model, EA and medication groups (=9 in each group). The IBS-D model with psychological abnormality was established by gavage of dinitrobenzene sulfonic acid (DNBS) + chronic restraint stress. EA (2 Hz/100 Hz, 0.3 mA) was applied to bilateral ST25 and ST37 for 20 min, once a day for 7 days. Rats of the medication group received gavage of pinaverium bromide solution (15 mg/kg), once a day for 7 days. The rats' food intake in 24 h, body mass and colonic contraction waves were recorded. The anhedonia-related behavior was measured using the sucrose consumption test. The elevated plus maze test (the open-arm residence time ratio) was used to assess the anxiety-like behavior. The small intestinal propulsion rate test was used to assess the intestinal motility. The expression levels of RhoA and ROCK proteins in the colonic tissue were measured by Western blot. RESULTS: After modeling, the body mass, food consumption, sucrose preference index, the open-arm residence time ratio andlatency of colonic contraction waves were significantly decreased (<0.05), and the number of contraction waves, intestinal propulsive rate, and the expression levels of RhoA and ROCK proteins considerably increased (<0.01，<0.05) in the model group relevant to the control group. Following the interventions, the decrease of body mass, food consumption, sucrose preference index, open-arm residence time ratio and latency of contraction waves, and the increase of the contraction waves, intestinal propulsive rate, and the expression levels of RhoA and ROCK proteins were all reversed by both EA and medication (<0.05，<0.01). The effect of EA was significantly superior to that of medication in increasing the sucrose pre-ference index (<0.05). CONCLUSION: EA can improve both colonic motility and psychological disorders in IBS-D rats with psychological disorder, which may be related to its function in down-regulating the expression of colonic RhoA and ROCK proteins.
目的：观察电针“天枢”（ST25）、“上巨虚”（ST37）对腹泻型肠易激综合征（IBS-D）大鼠胃肠动力、心理异常及RhoA和ROCK蛋白表达的影响，以探讨其改善IBS-D的潜在机制。 方法：将36只雄性大鼠随机等分为对照组、模型组、电针组和药物组（每组9只）。采用二硝基苯磺酸（DNBS）灌胃+慢性束缚应激建立伴有心理异常的IBS-D模型。电针双侧ST25、ST37（2Hz/100Hz，0.3mA），每次20分钟，每日1次，共7天。药物组大鼠灌胃匹维溴铵溶液（15mg/kg），每日1次，共7天。记录大鼠24小时摄食量、体质量及结肠收缩波。采用蔗糖消耗试验检测快感缺失相关行为。采用高架十字迷宫试验（开放臂停留时间比例）评估焦虑样行为。采用小肠推进率试验评估肠道动力。采用蛋白质免疫印迹法检测结肠组织中RhoA和ROCK蛋白的表达水平。 结果：造模后，模型组大鼠体质量、摄食量、蔗糖偏好指数、开放臂停留时间比例及结肠收缩波潜伏期均显著降低（P<0.05），收缩波次数、肠道推进率及RhoA和ROCK蛋白表达水平均显著升高（P<0.01，P<0.05）。干预后，电针和药物均能逆转模型组大鼠体质量、摄食量、蔗糖偏好指数、开放臂停留时间比例及收缩波潜伏期的降低，以及收缩波次数、肠道推进率及RhoA和ROCK蛋白表达水平的升高（P<0.05，P<0.01）。电针在提高蔗糖偏好指数方面的效果显著优于药物（P<0.05）。 结论：电针可改善伴有心理障碍的IBS-D大鼠的结肠动力和心理障碍，其机制可能与下调结肠RhoA和ROCK蛋白的表达有关。
Zhen Ci Yan Jiu. 2022-7-25
Zhongguo Zhen Jiu. 2022-12-12
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